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PMID: 19160517 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Holographic photolysis of caged neurotransmitters.

Nature methods ·Vol. 5 ·No. 9 ·2008-09-00 ·Pages 821-7

Lutz C, Otis TS, DeSars V, Charpak S, DiGregorio DA, Emiliani V

Abstract

Stimulation of light-sensitive chemical probes has become a powerful tool for the study of dynamic signaLing processes in living tissue. Classically, this approach has been constrained by limitations of lens-based and point-scanning illumination systems. Here we describe a microscope configuration that incorporates a nematic liquid-crystal spatial light modulator to generate holographic patterns of illumination. This microscope can produce illumination spots of variable size and number, and in patterns shaped to precisely match user-defined elements in a specimen. Using holographic illumination to photolyze caged glutamate in brain slices, we show that shaped excitation on segments of neuronal dendrites and simultaneous, multispot excitation of different dendrites enables precise spatial and rapid temporal control of glutamate receptor activation. By allowing the excitation volume shape to be tailored precisely, the holographic microscope provides an extremely flexible method for activation of various photosensitive proteins and small molecules.

MeSH Terms
Animals Brain/physiology Holography Photolysis Receptors, AMPA/physiology
Chemicals
Receptors, AMPA
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Lutz Christoph
Neurophysiology and New Microscopy Laboratory, Centre National de la Recherche Scientifique, Unité Mixte de Recherche 8154, Institut National de la Santé et de la Recherche Médicale, U603, Université Paris Descartes, Paris, France.
Otis Thomas S
DeSars Vincent
Charpak Serge
DiGregorio David A
Emiliani Valentina
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Article Info
Journal
Nature methods
Abbr.
Nat Methods
ISSN
1548-7091
DOI
10.1038/nmeth.1241
Published
2008-09-00
Pages
821-7
Language
English
Region
United States
NLM ID
101215604
PMCID
PMC2711023
Subset
IM
Grants
NICHD NIH HHS · R21 HD049238 · United States
NICHD NIH HHS · R21 HD049238-01 · United States
NICHD NIH HHS · R21 HD049238-02 · United States
NINDS NIH HHS · NS49238 · United States
External Links
PubMed source DOI: 10.1038/nmeth.1241 PMC Full Text View journal details
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