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PMID: 15673659 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Number and density of AMPA receptors in single synapses in immature cerebellum.

The Journal of neuroscience : the official journal of the Society for Neuroscience ·Vol. 25 ·No. 4 ·2005-01-26 ·Pages 799-807

Tanaka J, Matsuzaki M, Tarusawa E, Momiyama A, Molnar E, Kasai H, Shigemoto R

Abstract

The number of ionotropic receptors in synapses is an essential factor for determining the efficacy of fast transmission. We estimated the number of functional AMPA receptors at single postsynaptic sites by a combination of two-photon uncaging of glutamate and the nonstationary fluctuation analysis in immature rat Purkinje cells (PCs), which receive a single type of excitatory input from climbing fibers. Areas of postsynaptic membrane specialization at the recorded synapses were measured by reconstruction of serial ultrathin sections. The number of functional AMPA receptors was proportional to the synaptic area with a density of approximately 1280 receptors/microm2. Moreover, highly sensitive freeze-fracture replica labeling revealed a homogeneous density of immunogold particles for AMPA receptors in synaptic sites (910 +/- 36 particles/microm2) and much lower density in extrasynaptic sites (19 +/- 2 particles/microm2) in the immature PCs. Our results indicate that in this developing synapse, the efficacy of transmission is determined by the synaptic area.

MeSH Terms
Animals Cerebellum/growth & development,metabolism,ultrastructure Excitatory Postsynaptic Potentials/drug effects,physiology Freeze Fracturing/methods Glutamates Immunohistochemistry In Vitro Techniques Indoles Kinetics Purkinje Cells/physiology Rats Rats, Sprague-Dawley Receptors, AMPA/metabolism Synapses/metabolism,physiology
Chemicals
4-methoxy-7-nitroindolinyl-glutamate Glutamates Indoles Receptors, AMPA
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Tanaka Jun-ichi
Department of Physiological Sciences, Graduate University for Advanced Studies, Sokendai 444-8787, Japan.
Matsuzaki Masanori
Tarusawa Etsuko
Momiyama Akiko
Molnar Elek
Kasai Haruo
Shigemoto Ryuichi
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Article Info
Journal
The Journal of neuroscience : the official journal of the Society for Neuroscience
Abbr.
J Neurosci
ISSN
1529-2401
DOI
10.1523/JNEUROSCI.4256-04.2005
Published
2005-01-26
Pages
799-807
Language
English
Region
United States
NLM ID
8102140
PMCID
PMC6725634
Subset
IM
External Links
PubMed source DOI: 10.1523/JNEUROSCI.4256-04.2005 PMC Full Text View journal details
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