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PMID: 12626681 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Review

Agrobacterium-mediated plant transformation: the biology behind the "gene-jockeying" tool.

Microbiology and molecular biology reviews : MMBR ·Vol. 67 ·No. 1 ·2003-03-00 ·Pages 16-37, table of contents

Gelvin SB

Abstract

Agrobacterium tumefaciens and related Agrobacterium species have been known as plant pathogens since the beginning of the 20th century. However, only in the past two decades has the ability of Agrobacterium to transfer DNA to plant cells been harnessed for the purposes of plant genetic engineering. Since the initial reports in the early 1980s using Agrobacterium to generate transgenic plants, scientists have attempted to improve this "natural genetic engineer" for biotechnology purposes. Some of these modifications have resulted in extending the host range of the bacterium to economically important crop species. However, in most instances, major improvements involved alterations in plant tissue culture transformation and regeneration conditions rather than manipulation of bacterial or host genes. Agrobacterium-mediated plant transformation is a highly complex and evolved process involving genetic determinants of both the bacterium and the host plant cell. In this article, I review some of the basic biology concerned with Agrobacterium-mediated genetic transformation. Knowledge of fundamental biological principles embracing both the host and the pathogen have been and will continue to be key to extending the utility of Agrobacterium for genetic engineering purposes.

MeSH Terms
Biotechnology/methods Gene Expression Regulation Gene Silencing Gene Transfer Techniques Genes, Plant Genetic Engineering/methods Plants, Genetically Modified Rhizobium/genetics,pathogenicity Transformation, Genetic Transgenes Virulence/genetics
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Gelvin Stanton B
Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907-1392, USA. gelvin@bilbo.bio.purdue.edu
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Article Info
Journal
Microbiology and molecular biology reviews : MMBR
Abbr.
Microbiol Mol Biol Rev
ISSN
1092-2172
DOI
10.1128/MMBR.67.1.16-37.2003
Email
gelvin@bilbo.bio.purdue.edu
Published
2003-03-00
Pages
16-37, table of contents
Language
English
Region
United States
NLM ID
9706653
PMCID
PMC150518
Subset
IM
External Links
PubMed source DOI: 10.1128/MMBR.67.1.16-37.2003 PMC Full Text View journal details
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