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PMID: 8253677 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

The chromosomal virulence gene, chvE, of Agrobacterium tumefaciens is regulated by a LysR family member.

Journal of bacteriology ·Vol. 175 ·No. 24 ·1993-12-00 ·Pages 7880-6

Doty SL, Chang M, Nester EW

Abstract

Certain plant phenolic compounds and monosaccharides induce the transcription of virulence (vir) genes of Agrobacterium tumefaciens through the VirA-VirG two-component regulatory system. The product of the chromosomal virulence gene chvE is homologous to galactose-binding protein of Escherichia coli and is required for vir gene induction by sugars. Adjacent to, but divergent in transcription from, chvE is an open reading frame, now termed gbpR (galactose-binding protein regulator), that is homologous to the LysR family of transcriptional regulators. chvE::lacZ expression was induced by L-arabinose, D-galactose, and D-fucose when gbpR was present. In the absence of inducer, GbpR repressed chvE::lacZ expression. In addition, GbpR negatively regulated its own expression.

MeSH Terms
Agrobacterium tumefaciens/genetics,pathogenicity Bacterial Proteins/metabolism Base Sequence Calcium-Binding Proteins Carbohydrates/pharmacology Carrier Proteins/biosynthesis Chromosomes, Bacterial DNA Primers DNA, Bacterial/analysis,metabolism DNA-Binding Proteins Escherichia coli/genetics Gene Expression Regulation, Bacterial/drug effects Genes, Bacterial Molecular Sequence Data Monosaccharide Transport Proteins Periplasmic Binding Proteins Plasmids Polymerase Chain Reaction RNA, Messenger/metabolism Recombinant Fusion Proteins/biosynthesis Restriction Mapping Transcription Factors/metabolism Transcriptional Activation Virulence/genetics Virulence Factors beta-Galactosidase/biosynthesis
Chemicals
Bacterial Proteins Calcium-Binding Proteins Carbohydrates Carrier Proteins DNA Primers DNA, Bacterial DNA-Binding Proteins Monosaccharide Transport Proteins Periplasmic Binding Proteins RNA, Messenger Recombinant Fusion Proteins Transcription Factors Virulence Factors galactose-binding protein virG protein, Shigella flexneri LysR protein, Bacteria beta-Galactosidase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Doty S L
Department of Microbiology, University of Washington, Seattle 98195.
Chang M
Nester E W
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.175.24.7880-7886.1993
Published
1993-12-00
Pages
7880-6
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC206966
Subset
IM
Grants
NIGMS NIH HHS · 5-R01-GM32618-21 · United States
Databases
GENBANK
L10424
External Links
PubMed source DOI: 10.1128/jb.175.24.7880-7886.1993 PMC Full Text View journal details
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