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PMID: 1907267 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

rbcR [correction of rcbR], a gene coding for a member of the LysR family of transcriptional regulators, is located upstream of the expressed set of ribulose 1,5-bisphosphate carboxylase/oxygenase genes in the photosynthetic bacterium Chromatium vinosum.

Journal of bacteriology ·Vol. 173 ·No. 16 ·1991-08-00 ·Pages 5224-9

Viale AM, Kobayashi H, Akazawa T, Henikoff S

Abstract

An open reading frame, rbcR, was identified 226 bp upstream of rbcAB, i.e., the ribulose 1,5-bisphosphate carboxylase genes expressed in the phototrophic purple bacterium Chromatium vinosum. Several features reveal that rbcR encodes a member of the LysR family of transcriptional regulators, in which an anomalous content of lysine and arginine residues (Lys/Arg anomaly) was found. The expression of rbcR in Escherichia coli as a protein fused to the N-terminal region of beta-galactosidase led to reduced expression of rbcAB. Thus, rbcR is likely to encode a trans-acting transcriptional regulator of rbcAB expression in C. vinosum.

MeSH Terms
Amino Acid Sequence Arginine/genetics Bacterial Proteins Base Sequence Chromatium/enzymology,genetics Escherichia coli/metabolism Gene Expression Regulation, Bacterial/genetics Genes, Regulator/genetics Lysine/genetics Molecular Sequence Data Open Reading Frames/genetics Recombinant Fusion Proteins/biosynthesis Ribulose-Bisphosphate Carboxylase/genetics Sequence Alignment Transcription Factors/genetics
Chemicals
Bacterial Proteins Recombinant Fusion Proteins Transcription Factors RbcR protein, Bacteria Arginine Ribulose-Bisphosphate Carboxylase Lysine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Viale A M
Departamento de Microbiologia, Facultad de Ciencias Bioquimicas y Farmaceuticas, Universidad Nacional de Rosario, Argentina.
Kobayashi H
Akazawa T
Henikoff S
References (34)
34 references, click to expand
  1. Multiple sequence alignment with hierarchical clustering.
    Nucleic Acids Res. 1988 Nov 25;16(22):10881-90 PMID: 2849754
  2. Expression of genes for subunits of plant-type RuBisCO from Chromatium and production of the enzymically active molecule in Escherichia coli.
    FEBS Lett. 1985 Nov 18;192(2):283-8 PMID: 2998871
  3. Inducible cephalosporinase production in clinical isolates of Enterobacter cloacae is controlled by a regulatory gene that has been deleted from Escherichia coli.
    EMBO J. 1986 Dec 20;5(13):3709-14 PMID: 3030737
  4. Expression of cloned genes by in vivo insertion of tac promoter using a mini-Mu bacteriophage.
    Gene. 1988 May 30;65(2):305-14 PMID: 2970421
  5. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.
    Gene. 1985;33(1):103-19 PMID: 2985470
  6. Molecular and cellular regulation of autotrophic carbon dioxide fixation in microorganisms.
    Microbiol Rev. 1988 Jun;52(2):155-89 PMID: 3137427
  7. Common properties shared by growth-associated proteins of the regenerating optic nerve of goldfish (C. auratus).
    Neurosci Lett. 1988 Feb 29;85(2):267-71 PMID: 2453817
  8. Bacterial evolution.
    Microbiol Rev. 1987 Jun;51(2):221-71 PMID: 2439888
  9. Genetic and biochemical analysis of the MetR activator-binding site in the metE metR control region of Salmonella typhimurium.
    J Bacteriol. 1989 Oct;171(10):5620-9 PMID: 2676984
  10. DNA footprint analysis of the transcriptional activator proteins NodD1 and NodD3 on inducible nod gene promoters.
    J Bacteriol. 1989 Oct;171(10):5492-502 PMID: 2793828
  11. Characterization of Acinetobacter calcoaceticus catM, a repressor gene homologous in sequence to transcriptional activator genes.
    J Bacteriol. 1989 Oct;171(10):5410-21 PMID: 2793826
  12. Demonstration, characterization, and mutational analysis of NahR protein binding to nah and sal promoters.
    J Bacteriol. 1989 Feb;171(2):837-46 PMID: 2914873
  13. A chromosomal Agrobacterium tumefaciens gene required for effective plant signal transduction.
    J Bacteriol. 1990 Apr;172(4):1814-22 PMID: 2156804
  14. Identification and characterization of a gene that controls colony morphology and auto-aggregation in Escherichia coli K12.
    J Gen Microbiol. 1990 Mar;136(3):455-62 PMID: 2167922
  15. Nucleotide sequencing and characterization of Pseudomonas putida catR: a positive regulator of the catBC operon is a member of the LysR family.
    J Bacteriol. 1990 Feb;172(2):922-31 PMID: 1688844
  16. Improved detection of helix-turn-helix DNA-binding motifs in protein sequences.
    Nucleic Acids Res. 1990 Sep 11;18(17):5019-26 PMID: 2402433
  17. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid.
    J Bacteriol. 1978 Jun;134(3):1141-56 PMID: 149110
  18. Arginyl residues and anion binding sites in proteins.
    Mol Cell Biochem. 1979 Jul 31;26(2):71-92 PMID: 388184
  19. Finding protein similarities with nucleotide sequence databases.
    Methods Enzymol. 1990;183:111-32 PMID: 2314271
  20. Biosynthetic mechanism of ribulose-1,5-bisphosphate carboxylase in the purple photosynthetic bacterium, Chromatium vinosum.
    Arch Biochem Biophys. 1982 Apr 1;214(2):531-9 PMID: 6807199
  21. DNA structure and gene regulation.
    Prog Nucleic Acid Res Mol Biol. 1980;24:167-267 PMID: 7005967
  22. Nucleic acid sequence database IV.
    DNA. 1982;1(4):365-74 PMID: 7169012
  23. Sequence and expression of genes encoding the large and small subunits of ribulose 1,5-bisphosphate carboxylase/oxygenase from Chromatium vinosum.
    Gene. 1991 Jan 2;97(1):55-62 PMID: 1899846
  24. Identification and organization of carbon dioxide fixation genes in Xanthobacter flavus H4-14.
    Mol Gen Genet. 1991 Feb;225(2):320-30 PMID: 1900916
  25. Distinct properties of Escherichia coli products of plant-type ribulose-1,5-bisphosphate carboxylase/oxygenase directed by two sets of genes from the photosynthetic bacterium Chromatium vinosum.
    J Biol Chem. 1990 Oct 25;265(30):18386-92 PMID: 2211708
  26. Positive transcriptional regulation of an iron-regulated virulence gene in Vibrio cholerae.
    Proc Natl Acad Sci U S A. 1991 Feb 15;88(4):1125-9 PMID: 1705025
  27. A large family of bacterial activator proteins.
    Proc Natl Acad Sci U S A. 1988 Sep;85(18):6602-6 PMID: 3413113
  28. The Escherichia coli regulatory protein OxyR discriminates between methylated and unmethylated states of the phage Mu mom promoter.
    EMBO J. 1989 Aug;8(8):2403-10 PMID: 2551682
  29. Positive regulation of glutamate biosynthesis in Bacillus subtilis.
    J Bacteriol. 1989 Sep;171(9):4718-27 PMID: 2548995
  30. Transcriptional activation at adjacent operators in the divergent-overlapping ilvY and ilvC promoters of Escherichia coli.
    J Mol Biol. 1988 Oct 5;203(3):643-63 PMID: 3062177
  31. Transcriptional regulation of genes for plant-type ribulose-1,5-bisphosphate carboxylase/oxygenase in the photosynthetic bacterium, Chromatium vinosum.
    Eur J Biochem. 1988 May 2;173(3):483-9 PMID: 3286254
  32. Expressed genes for plant-type ribulose 1,5-bisphosphate carboxylase/oxygenase in the photosynthetic bacterium Chromatium vinosum, which possesses two complete sets of the genes.
    J Bacteriol. 1989 May;171(5):2391-400 PMID: 2708310
  33. Mechanism and control of transcription initiation in prokaryotes.
    Annu Rev Biochem. 1985;54:171-204 PMID: 3896120
  34. Nucleotide sequence of the Salmonella typhimurium metR gene and the metR-metE control region.
    J Bacteriol. 1987 Sep;169(9):3932-7 PMID: 3040668
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.173.16.5224-5229.1991
Published
1991-08-00
Pages
5224-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC208217
Subset
IM
Databases
GENBANK
M64032, M64121, M64122, M64123, M64124, M64125, M64126, M64127, M64128, M64129
External Links
PubMed source DOI: 10.1128/jb.173.16.5224-5229.1991 PMC Full Text View journal details
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