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PMID: 1900916 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Identification and organization of carbon dioxide fixation genes in Xanthobacter flavus H4-14.

Molecular & general genetics : MGG ·Vol. 225 ·No. 2 ·1991-02-00 ·Pages 320-30

Meijer WG, Arnberg AC, Enequist HG, Terpstra P, Lidstrom ME, Dijkhuizen L

Abstract

The genes encoding the large (cfxL) and small (cfxS) subunits of ribulose-1,5-bisphosphate carboxylase (RuBisC/O) from Xanthobacter flavus H4-14 were identified and characterized. The RuBisC/O genes are separated by 11 bp and cotranscribed in Escherichia coli from the lac promoter in the order cfxLS. Primer extension and R-loop experiments with RNA isolated from autotrophically grown X. flavus H4-14 showed that transcription of cfxL and cfxS initiated 22 bp upstream from cfxL and resulted in a mRNA of at least 2.3 kb. DNA sequence analysis identified the start of an open reading frame transcribed divergently from cfxL, and displaying significant similarities with genes belonging to the lysR family of transcriptional activators. Downstream from cfxS an additional open reading frame was identified with unknown function. Expression studies showed that the genes encoding fructosebisphosphatase (cfxF) and phosphoribulokinase (cfxP) are located downstream from cfxLS. The cfxF and cfxP genes are cotranscribed in the same direction as cfxLS in the order cfxFP.

Related Genes
cfxF cfxLS cfxP
MeSH Terms
Amino Acid Sequence Base Sequence Carbon Dioxide/metabolism Chromosome Mapping Chromosomes, Bacterial Genes, Bacterial Gram-Negative Aerobic Bacteria/enzymology,genetics,ultrastructure Kinetics Microscopy, Electron Molecular Sequence Data Open Reading Frames Promoter Regions, Genetic Restriction Mapping Ribulose-Bisphosphate Carboxylase/genetics,metabolism Sequence Homology, Nucleic Acid Transcription, Genetic
Chemicals
Carbon Dioxide Ribulose-Bisphosphate Carboxylase
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Meijer W G
Department of Microbiology, University of Groningen, The Netherlands.
Arnberg A C
Enequist H G
Terpstra P
Lidstrom M E
Dijkhuizen L
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00269865
Published
1991-02-00
Pages
320-30
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Grants
NIGMS NIH HHS · GM36296-04 · United States
Databases
GENBANK
M64566, S63796, S63798, S79268, S79270, S79277, S85222, S85224, X17252, X57666
External Links
PubMed source DOI: 10.1007/BF00269865
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