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PMID: 8871559 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

FLP-mediated recombination of FRT sites in the maize genome.

Nucleic acids research ·Vol. 24 ·No. 19 ·1996-10-01 ·Pages 3784-9

Lyznik LA, Rao KV, Hodges TK

Abstract

Molecular evidence is provided for genomic recombinations in maize cells induced by the yeast FLP/FRT site-specific recombination system. The FLP protein recombined FRT sites previously integrated into the maize genome leading to excision of a selectable marker, the neo gene. NPTII activity was not observed after the successful recombination process; instead, the gusA gene was activated by the removal of the blocking DNA fragment. Genomic sequencing in the region of the FRT site (following the recombination reaction) indicated that a precise rearrangement of genomic DNA sequences had taken place. The functional FLP gene could be either expressed transiently or after stable integration into the maize genome. The efficiency of genomic recombinations was high enough that a selection for recombination products, or for FLP expression, was not required. The results presented here establish the FLP/FRT site-specific recombination system as an important tool for controlled modifications of maize genomic DNA.

MeSH Terms
Base Sequence Cloning, Molecular DNA Nucleotidyltransferases/genetics,metabolism DNA, Plant Fungal Proteins/metabolism Genetic Markers Genetic Vectors Genome, Plant Glucuronidase/genetics Molecular Sequence Data Recombination, Genetic Zea mays/genetics
Chemicals
DNA, Plant Fungal Proteins Genetic Markers DNA Nucleotidyltransferases FLP recombinase Glucuronidase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Lyznik L A
Department of Botany and Plant Pathology, Purdue University, West Lafayette, IN 47907, USA.
Rao K V
Hodges T K
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/24.19.3784
Published
1996-10-01
Pages
3784-9
Language
English
Region
England
NLM ID
0411011
PMCID
PMC146161
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/24.19.3784 PMC Full Text View journal details
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