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PMID: 8650242 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Flp recombinase promotes site-specific DNA recombination in embryonic stem cells and transgenic mice.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 93 ·No. 12 ·1996-06-11 ·Pages 6191-6

Dymecki SM

Abstract

Site-specific recombinases are being developed as tools for "in vivo" genetic engineering because they can catalyze precise excisions, integrations, inversions, or translocations of DNA between their distinct recognition target sites. Here it is demonstrated that Flp recombinase can effectively mediate site-specific excisional recombination in mouse embryonic stem cells, in differentiating embryonal carcinoma cells, and in transgenic mice. Broad Flp expression is compatible with normal development, suggesting that Flp can be used to catalyze recombination in most cell types. These properties indicate that Flp can be exploited to make prescribed alterations in the mouse genome.

MeSH Terms
Animals Base Sequence Cell Differentiation Cell Line DNA/metabolism DNA Nucleotidyltransferases/metabolism Embryo, Mammalian/cytology,metabolism Embryonal Carcinoma Stem Cells Germ Cells Mice Mice, Transgenic Molecular Sequence Data Neoplastic Stem Cells Recombination, Genetic Stem Cells/metabolism Transgenes
Chemicals
DNA DNA Nucleotidyltransferases FLP recombinase
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Dymecki S M
Department of Embryology, Carnegie Institution of Washington, Baltimore, MD 21210, USA.
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36 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.93.12.6191
Published
1996-06-11
Pages
6191-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC39212
Subset
IM
Grants
NICHD NIH HHS · N01-HD-0-2911 · United States
NICHD NIH HHS · R01-HD-30830 · United States
External Links
PubMed source DOI: 10.1073/pnas.93.12.6191 PMC Full Text View journal details
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