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PMID: 2011519 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A bacterial model system for chromosomal targeting.

Nucleic acids research ·Vol. 19 ·No. 3 ·1991-02-11 ·Pages 443-8

Huang LC, Wood EA, Cox MM

Abstract

A system that permits efficient site-specific chromosomal targeting of foreign DNA on the Escherichia coli chromosome has been developed, using the FLP site-specific recombination system derived from the yeast 2 mu plasmid. The system demonstrates the feasibility of using site-specific recombination for this purpose, and provides a means to gather information on parameters that may affect chromosomal targeting to guide efforts to establish similar systems in higher eukaryotes. In this model system, the efficiency of integration of foreign DNA is affected by the location of the target site in the chromosome, and the structure of the recombination sites.

MeSH Terms
Base Sequence Blotting, Southern Chromosomes, Bacterial/physiology,ultrastructure DNA Nucleotidyltransferases/metabolism DNA, Bacterial/genetics Escherichia coli/genetics Genetic Engineering/methods Genetic Vectors Molecular Sequence Data Recombination, Genetic
Chemicals
DNA, Bacterial DNA Nucleotidyltransferases FLP recombinase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Huang L C
Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin-Madison 53706.
Wood E A
Cox M M
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21 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/19.3.443
Published
1991-02-11
Pages
443-8
Language
English
Region
England
NLM ID
0411011
PMCID
PMC333631
Subset
IM
Grants
NIGMS NIH HHS · GM37835 · United States
External Links
PubMed source DOI: 10.1093/nar/19.3.443 PMC Full Text View journal details
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