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PMID: 8152416 Published · ppublish English Journal Article

Functional expression of the yeast FLP/FRT site-specific recombination system in Nicotiana tabacum.

Molecular & general genetics : MGG ·Vol. 242 ·No. 6 ·1994-03-00 ·Pages 653-7

Lloyd AM, Davis RW

Abstract

The FLP/FRT site-specific recombination system of Saccharomyces cerevisiae was expressed in stably transformed tobacco plants. The FLP protein efficiently catalyzes recombination between two directly repeated FLP recombination target (FRT) sites, deleting the sequence between them. In the constructs tested here, this deletion places the CaMV 35S promoter adjacent to a hygromycin resistance marker; transcriptional activation of the marker allows direct selection of recombination events. After crossing plants containing an integrated FLP expression construct with plants containing a FLP substrate, F1 seedlings can be selected directly for hygromycin resistance, indicating that recombination occurs at, or very early after zygote formation. Molecular analysis confirmed the expected recombination product.

MeSH Terms
Agrobacterium tumefaciens/genetics Base Sequence DNA Nucleotidyltransferases/genetics DNA Primers/genetics Fungal Proteins/genetics Gene Expression Genes, Fungal Genetic Vectors Molecular Sequence Data Plants, Genetically Modified Plants, Toxic Recombination, Genetic Saccharomyces cerevisiae/genetics Tobacco/genetics
Chemicals
DNA Primers Fungal Proteins DNA Nucleotidyltransferases FLP recombinase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Lloyd A M
Department of Biochemistry, Stanford University, CA 94305.
Davis R W
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00283419
Published
1994-03-00
Pages
653-7
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00283419
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