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PMID: 8127869 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Cre recombinase-mediated site-specific recombination between plant chromosomes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 91 ·No. 5 ·1994-03-01 ·Pages 1706-10

Qin M, Bayley C, Stockton T, Ow DW

Abstract

We report the use of the bacteriophage P1 Cre-lox system for generating conservative site-specific recombination between tobacco chromosomes. Two constructs, one containing a promoterless hygromycin-resistance gene preceded by a lox site (lox-hpt) and the other containing a cauliflower mosaic virus 35S promoter linked to a lox sequence and the cre coding region (35S-lox-cre), were introduced separately into tobacco plants. Crosses between plants harboring either construct produced plants with the two constructs situated on different chromosomes. Plants with recombination events were identified by selecting for hygromycin resistance, a phenotype expressed upon recombination. Molecular analysis showed that these recombination events occurred specifically at the lox sites and resulted in the reciprocal exchange of flanking host DNA. Progenies of these plants showed 67-100% cotransmission of the new transgenes, 35S-lox-hpt and lox-cre, consistent with the preferential cosegregation of translocated chromosomes. These results illustrate that site-specific recombination systems can be useful tools for the large-scale manipulation of eukaryotic chromosomes in vivo.

Related Genes
cre hpt lox
MeSH Terms
Bacteriophage P1/enzymology,genetics Base Sequence Caulimovirus/genetics Chromosomes Cinnamates DNA Nucleotidyltransferases/genetics DNA Primers/genetics Drug Resistance/genetics Gene Rearrangement Genetic Vectors Hygromycin B/analogs & derivatives,pharmacology Integrases Molecular Sequence Data Plants, Genetically Modified Plants, Toxic Promoter Regions, Genetic Recombination, Genetic Tobacco/genetics Viral Proteins
Chemicals
Cinnamates DNA Primers Viral Proteins Hygromycin B hygromycin A Cre recombinase DNA Nucleotidyltransferases Integrases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Qin M
Plant Gene Expression Center, U.S. Department of Agriculture, Albany, CA 94710.
Bayley C
Stockton T
Ow D W
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21 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.91.5.1706
Published
1994-03-01
Pages
1706-10
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC43232
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.91.5.1706 PMC Full Text View journal details
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