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PMID: 1502156 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The T-DNA-linked VirD2 protein contains two distinct functional nuclear localization signals.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 89 ·No. 16 ·1992-08-15 ·Pages 7442-6

Tinland B, Koukolíková-Nicola Z, Hall MN, Hohn B

Abstract

Agrobacterium tumefaciens causes neoplastic growth in plants by transferring a piece of DNA, called T-DNA, into the nucleus of the plant cell. The virulence protein VirD2 of A. tumefaciens is tightly linked to the T-DNA and is thought to direct it to the plant genome. Here we show that the VirD2 protein contains two nuclear localization signals that are functional both in yeast and in plant cells. One signal is located in the N-terminal part of the protein and resembles a single-cluster-type nuclear localization signal. The second signal is near the C terminus and is a bipartite-type nuclear localization signal. The involvement of these sequences in the entry of the T-DNA into the nucleus is discussed.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/analysis,genetics,metabolism Base Sequence Cell Nucleus/physiology Cloning, Molecular DNA, Bacterial/metabolism Escherichia coli/genetics Fluorescent Antibody Technique Molecular Sequence Data Oligodeoxyribonucleotides Plants, Toxic Recombinant Fusion Proteins/analysis,metabolism Rhizobium/genetics,metabolism Saccharomyces cerevisiae/genetics Tobacco/microbiology Virulence Factors beta-Galactosidase/analysis,metabolism
Chemicals
Bacterial Proteins DNA, Bacterial Oligodeoxyribonucleotides Recombinant Fusion Proteins T-DNA Virulence Factors beta-Galactosidase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Tinland B
Friedrich Miescher-Institut, Basel, Switzerland.
Koukolíková-Nicola Z
Hall M N
Hohn B
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37 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.89.16.7442
Published
1992-08-15
Pages
7442-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC49726
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.89.16.7442 PMC Full Text View journal details
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