Home LiteratureArticle Details
PMID: 3005818 Published · ppublish English Journal Article Review

Uses of lac fusions for the study of biological problems.

Microbiological reviews ·Vol. 49 ·No. 4 ·1985-12-00 ·Pages 398-418

Silhavy TJ, Beckwith JR

Abstract

暂无摘要

MeSH Terms
Amino Acid Sequence Bacillus subtilis/genetics Bacteriophages/genetics Cloning, Molecular DNA Transposable Elements Gene Expression Regulation Genes, Regulator Genetic Vectors Hybridization, Genetic Lac Operon Phenotype Plasmids Protein Biosynthesis Proteins/analysis Transcription, Genetic
Chemicals
DNA Transposable Elements Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Silhavy T J
Beckwith J R
References (121)
121 references, click to expand
  1. Effect of suppressors of SOS-mediated filamentation on sfiA operon expression in Escherichia coli.
    J Bacteriol. 1983 Jan;153(1):169-75 PMID: 6336731
  2. Point mutations that reduce the expression of malPQ, a positively controlled operon of Escherichia coli.
    J Mol Biol. 1984 Jul 25;177(1):69-86 PMID: 6379197
  3. Use of gene fusions to isolate promoter mutants in the transfer RNA gene tyrT of Escherichia coli.
    J Mol Biol. 1979 May 25;130(3):303-15 PMID: 381676
  4. Translational control of IS10 transposition.
    Cell. 1983 Sep;34(2):683-91 PMID: 6311438
  5. The expression in yeast of the Escherichia coli galK gene on CYC1::galK fusion plasmids.
    Gene. 1983 Nov;25(2-3):249-62 PMID: 6198241
  6. The nature of lactose operator constitive mutations.
    J Mol Biol. 1971 Jul 28;59(2):273-305 PMID: 4935786
  7. Interaction of the plasmid R6K-encoded replication initiator protein with its binding sites on DNA.
    Cell. 1983 Aug;34(1):125-34 PMID: 6224568
  8. The replication initiator protein of plasmid R6K tagged with beta-galactosidase shows sequence-specific DNA-binding.
    Cell. 1983 Jan;32(1):131-40 PMID: 6297781
  9. Regulation of proline utilization in Salmonella typhimurium: characterization of put::Mu d(Ap, lac) operon fusions.
    J Bacteriol. 1983 May;154(2):561-8 PMID: 6302076
  10. Use of gene fusions to determine a partial signal sequence of alkaline phosphatase.
    J Bacteriol. 1979 Sep;139(3):932-9 PMID: 113391
  11. A mutation distal to the messenger RNA endpoint reduces transcription termination in the tryptophan operon in Escherichia coli.
    J Mol Biol. 1979 Sep 5;133(1):189-97 PMID: 393832
  12. Antibody-mediated activation of a deletion-mutant beta-galactosidase defective in the alpha region.
    FEBS Lett. 1976 Sep 1;67(3):299-302 PMID: 786724
  13. Targeting of E. coli beta-galactosidase to the nucleus in yeast.
    Cell. 1984 Apr;36(4):1057-65 PMID: 6323016
  14. General method, using Mu-Mud1 dilysogens, to determine the direction of transcription of and generate deletions in the glnA region of Escherichia coli.
    J Bacteriol. 1981 Apr;146(1):260-8 PMID: 6111550
  15. Intracellular targeting and import of an F1-ATPase beta-subunit-beta-galactosidase hybrid protein into yeast mitochondria.
    Proc Natl Acad Sci U S A. 1984 Jul;81(13):3983-7 PMID: 6330727
  16. Mechanisms of protein localization.
    Microbiol Rev. 1983 Sep;47(3):313-44 PMID: 6355805
  17. Direct selection of mutations reducing transcription or translation of the recA gene of Escherichia coli with a recA-lacZ protein fusion.
    J Bacteriol. 1985 Aug;163(2):748-55 PMID: 3160689
  18. Regulation of fatty acid transport in Escherichia coli: analysis by operon fusion.
    J Bacteriol. 1983 Sep;155(3):1450-4 PMID: 6350271
  19. The nucleotide sequence of the gene for malF protein, an inner membrane component of the maltose transport system of Escherichia coli. Repeated DNA sequences are found in the malE-malF intercistronic region.
    J Biol Chem. 1984 Sep 10;259(17):10896-903 PMID: 6088520
  20. A DELETION ANALYSIS OF THE LAC OPERATOR REGION IN ESCHERICHIA COLI.
    J Mol Biol. 1964 Mar;8:427-30 PMID: 14168696
  21. Beta-galactosidase gene fusions for analyzing gene expression in escherichia coli and yeast.
    Methods Enzymol. 1983;100:293-308 PMID: 6312261
  22. Phosphate-controlled gene expression in Escherichia coli K12 using Mudl-directed lacZ fusions.
    J Mol Biol. 1982 Jul 5;158(3):347-63 PMID: 6813506
  23. A unique mechanism regulating gene expression: translational inhibition by a complementary RNA transcript (micRNA).
    Proc Natl Acad Sci U S A. 1984 Apr;81(7):1966-70 PMID: 6201848
  24. Mutations that alter the DNA sequence specificity of the catabolite gene activator protein of E. coli.
    Nature. 1984 Sep 20-26;311(5983):232-5 PMID: 6090927
  25. A new class of promoter mutations in the lactose operon of Escherichia coli.
    J Mol Biol. 1974 Aug 25;87(4):715-24 PMID: 4372362
  26. The galactose operon of E. coli K-12. II. A deletion analysis of operon structure and polarity.
    Genetics. 1969 Jun;62(2):249-64 PMID: 4905872
  27. Fusions of secreted proteins to alkaline phosphatase: an approach for studying protein secretion.
    Proc Natl Acad Sci U S A. 1985 Aug;82(15):5107-11 PMID: 3860846
  28. Analysis of gene control signals by DNA fusion and cloning in Escherichia coli.
    J Mol Biol. 1980 Apr;138(2):179-207 PMID: 6997493
  29. Analysis of regulation of phoB expression using a phoB-cat fusion.
    J Bacteriol. 1983 Nov;156(2):710-7 PMID: 6313614
  30. An immunologically active chimaeric protein containing herpes simplex virus type 1 glycoprotein D.
    Nature. 1983 Mar 3;302(5903):72-4 PMID: 6298636
  31. In vivo formation of gene fusions encoding hybrid beta-galactosidase proteins in one step with a transposable Mu-lac transducing phage.
    Proc Natl Acad Sci U S A. 1984 Jan;81(2):535-9 PMID: 6320194
  32. Dominant constitutive mutations in malT, the positive regulator gene of the maltose regulon in Escherichia coli.
    J Mol Biol. 1978 Sep 15;124(2):359-71 PMID: 101676
  33. Identification of the malK gene product. A peripheral membrane component of the Escherichia coli maltose transport system.
    J Biol Chem. 1981 Jan 25;256(2):560-2 PMID: 6778869
  34. Lambda placMu: a transposable derivative of bacteriophage lambda for creating lacZ protein fusions in a single step.
    J Bacteriol. 1984 Jun;158(3):1084-93 PMID: 6327627
  35. Construction, isolation and implications of repressor-galactosidase - beta-galactosidase hybrid molecules.
    Eur J Biochem. 1977 Oct 3;79(2):381-6 PMID: 411650
  36. Deletion map of the Escherichia coli structural gene for alkaline phosphatase, phoA.
    J Bacteriol. 1981 Jan;145(1):288-92 PMID: 6450745
  37. New heat shock puffs and beta-galactosidase activity resulting from transformation of Drosophila with an hsp70-lacZ hybrid gene.
    Cell. 1983 Dec;35(2 Pt 1):403-10 PMID: 6418386
  38. Exogenous induction of the Escherichia coli hexose phosphate transport system defined by uhp-lac operon fusions.
    J Bacteriol. 1981 Oct;148(1):203-9 PMID: 6793554
  39. Prophage lambda at unusual chromosomal locations. II. Mutations induced by bacteriophage lambda in Escherichia coli K12.
    J Mol Biol. 1973 Oct 25;80(2):297-314 PMID: 4587404
  40. Amino acid sequence homology among the major outer membrane proteins of Escherichia coli.
    Proc Natl Acad Sci U S A. 1984 Feb;81(4):1048-52 PMID: 6366795
  41. Position of the mutation in beta-galactosidase ochre mutant U118.
    J Bacteriol. 1978 Jan;133(1):437-8 PMID: 412841
  42. Fusions of the lac and trp Regions of the Escherichia coli Chromosome.
    J Bacteriol. 1970 Dec;104(3):1273-9 PMID: 16559103
  43. An active cistron fragment.
    J Mol Biol. 1962 Apr;4:288-92 PMID: 13877974
  44. Mechanism of incorporation of cell envelope proteins in Escherichia coli.
    Annu Rev Microbiol. 1982;36:435-65 PMID: 6756294
  45. Construction of Tn5 lac, a transposon that fuses lacZ expression to exogenous promoters, and its introduction into Myxococcus xanthus.
    Proc Natl Acad Sci U S A. 1984 Sep;81(18):5816-20 PMID: 6091110
  46. Invertase signal and mature sequence substitutions that delay intercompartmental transport of active enzyme.
    J Cell Biol. 1985 May;100(5):1664-75 PMID: 3886671
  47. Transposable lambda placMu bacteriophages for creating lacZ operon fusions and kanamycin resistance insertions in Escherichia coli.
    J Bacteriol. 1985 Jun;162(3):1092-9 PMID: 2987183
  48. Sequence information within the lamB genes in required for proper routing of the bacteriophage lambda receptor protein to the outer membrane of Escherichia coli K-12.
    J Mol Biol. 1982 Mar 25;156(1):93-112 PMID: 6212690
  49. Use of phi(glp-lac) in studies of respiratory regulation of the Escherichia coli anaerobic sn-glycerol-3-phosphate dehydrogenase genes (glpAB).
    J Bacteriol. 1984 Feb;157(2):591-8 PMID: 6363389
  50. Structure of the malB region in Escherichia coli K12. II. Genetic map of the malE,F,G operon.
    Mol Gen Genet. 1979 Jul 24;174(3):249-59 PMID: 384167
  51. Selection for mutants altered in the expression or export of outer membrane porin OmpF.
    J Bacteriol. 1985 Jun;162(3):1047-53 PMID: 2987179
  52. Open reading frame cloning: identification, cloning, and expression of open reading frame DNA.
    Proc Natl Acad Sci U S A. 1982 Nov;79(21):6598-602 PMID: 6815653
  53. Genetic mapping and transcriptional orientation of the fimD gene.
    J Bacteriol. 1983 Dec;156(3):1052-8 PMID: 6139362
  54. Enhanced expression of cro-beta-galactosidase fusion proteins under the control of the PR promoter of bacteriophage lambda.
    EMBO J. 1982;1(10):1217-24 PMID: 6327257
  55. A change from nonsense to sense in the genetic code.
    Proc Natl Acad Sci U S A. 1962 Jul 15;48:1114-21 PMID: 13867417
  56. Regulation of outer membrane protein synthesis in Escherichia coli K-12: deletion of ompC affects expression of the OmpF protein.
    J Bacteriol. 1984 Aug;159(2):555-63 PMID: 6086576
  57. Intragenic regions required for LamB export.
    Proc Natl Acad Sci U S A. 1984 Jun;81(12):3830-4 PMID: 6374667
  58. Methylation of single-stranded DNA in vitro introduces new restriction endonuclease cleavage sites.
    Nature. 1978 Mar 23;272(5651):375-7 PMID: 416358
  59. In vitro construction and characterization of phoA-lacZ gene fusions in Escherichia coli.
    J Bacteriol. 1983 Apr;154(1):356-65 PMID: 6403507
  60. The spc ribosomal protein operon of Escherichia coli: sequence and cotranscription of the ribosomal protein genes and a protein export gene.
    Nucleic Acids Res. 1983 May 11;11(9):2599-616 PMID: 6222285
  61. A system to study promoter and terminator signals recognized by Escherichia coli RNA polymerase.
    Gene Amplif Anal. 1981;2:383-415 PMID: 6101056
  62. Regulation of the regulatory gene for the arabinose pathway, araC.
    J Mol Biol. 1976 Jul 5;104(3):557-66 PMID: 781294
  63. New regulatory mutations affecting the expression of the threonine operon in Escherichia coli K-12.
    Mol Gen Genet. 1978 Jun 1;162(1):95-100 PMID: 97515
  64. Genetic analysis of protein export in Escherichia coli K12.
    Annu Rev Biochem. 1985;54:101-34 PMID: 3896116
  65. Use of bacteriophage transposon Mu d1 to determine the orientation for three proC-linked phosphate-starvation-inducible (psi) genes in Escherichia coli K-12.
    J Bacteriol. 1981 Apr;146(1):93-101 PMID: 6260750
  66. beta-Galactosidase alpha-complementation. A model of protein-protein interaction.
    Mol Cell Biochem. 1982 Nov 26;49(2):87-96 PMID: 6818452
  67. Sites within gene lacZ of Escherichia coli for formation of active hybrid beta-galactosidase molecules.
    J Bacteriol. 1979 Jul;139(1):13-8 PMID: 110776
  68. Invertase beta-galactosidase hybrid proteins fail to be transported from the endoplasmic reticulum in Saccharomyces cerevisiae.
    Mol Cell Biol. 1984 Nov;4(11):2347-55 PMID: 6440005
  69. Cloned structural gene (ompA) for an integral outer membrane protein of Escherichia coli K-12: localization on hybrid plasmid pTU100 and expression of a fragment of the gene.
    Mol Gen Genet. 1980;179(1):13-20 PMID: 6256604
  70. Overproduction of the Tn3 transposition protein and its role in DNA transposition.
    Cell. 1982 Feb;28(2):345-54 PMID: 6277510
  71. A previously unidentified gene in the spc operon of Escherichia coli K12 specifies a component of the protein export machinery.
    Cell. 1982 Nov;31(1):227-35 PMID: 6297749
  72. Fusion of Escherichia coli lacZ to the cytochrome c gene of Saccharomyces cerevisiae.
    Proc Natl Acad Sci U S A. 1981 Apr;78(4):2199-203 PMID: 6264467
  73. Mutations affecting localization of an Escherichia coli outer membrane protein, the bacteriophage lambda receptor.
    J Mol Biol. 1980 Jul 25;141(1):63-90 PMID: 6448927
  74. Osmotic control of kdp operon expression in Escherichia coli.
    Proc Natl Acad Sci U S A. 1981 Jan;78(1):464-8 PMID: 6787588
  75. Improved methods for maximizing expression of a cloned gene: a bacterium that synthesizes rabbit beta-globin.
    Cell. 1980 Jun;20(2):543-53 PMID: 6248249
  76. Regulation of expression of the flagellin gene (hag) in Escherichia coli K-12: analysis of hag-lac gene fusions.
    J Bacteriol. 1979 Sep;139(3):721-9 PMID: 113385
  77. Immunoenzymatic detection of expressed gene fragments cloned in the lac Z gene of E. coli.
    EMBO J. 1982;1(4):509-12 PMID: 6329687
  78. Isolation and characterization of regulatory mutations affecting the expression of the guaBA operon of Escherichia coli K-12.
    Mol Gen Genet. 1984;195(1-2):77-82 PMID: 6387393
  79. Yeast genes fused to beta-galactosidase in Escherichia coli can be expressed normally in yeast.
    Proc Natl Acad Sci U S A. 1981 Apr;78(4):2460-4 PMID: 6787605
  80. Genetic fusions defining trp and lac operon regulatory elements.
    J Mol Biol. 1975 Apr 15;93(3):331-50 PMID: 1095760
  81. Genetic analysis of protein export in Escherichia coli.
    Methods Enzymol. 1983;97:3-11 PMID: 6419017
  82. Selection of lac gene fusions in vivo: ompR-lacZ fusions that define a functional domain of the ompR gene product.
    J Bacteriol. 1984 Aug;159(2):750-6 PMID: 6086585
  83. Mutations that define the promoter of ompF, a gene specifying a major outer membrane porin protein.
    J Bacteriol. 1985 Jun;162(3):1054-60 PMID: 2987180
  84. Mutations in the promoter regions of the malEFG and malK-lamB operons of Escherichia coli K12.
    J Mol Biol. 1983 Nov 15;170(4):861-82 PMID: 6417341
  85. The synthesis of export-defective proteins can interfere with normal protein export in Escherichia coli.
    J Biol Chem. 1984 Oct 10;259(19):12193-200 PMID: 6384220
  86. Transposition of the Lac region of E. coli.
    Cold Spring Harb Symp Quant Biol. 1966;31:393-401 PMID: 4866389
  87. Translational coupling of the trpB and trpA genes in the Escherichia coli tryptophan operon.
    J Bacteriol. 1984 Feb;157(2):363-7 PMID: 6319355
  88. Genetic engineering in vivo using translocatable drug-resistance elements. New methods in bacterial genetics.
    J Mol Biol. 1977 Oct 15;116(1):125-59 PMID: 338917
  89. Transcription termination at the end of the tryptophan operon of Escherichia coli.
    J Mol Biol. 1977 May 25;112(3):423-36 PMID: 327077
  90. A mechanism for repressor action.
    J Mol Biol. 1969 Jul 14;43(1):201-13 PMID: 4897791
  91. Labeling of proteins with beta-galactosidase by gene fusion. Identification of a cytoplasmic membrane component of the Escherichia coli maltose transport system.
    J Biol Chem. 1980 Jan 10;255(1):168-74 PMID: 6765939
  92. Isolation and characterization of delta ompB strains of Escherichia coli by a general method based on gene fusions.
    J Bacteriol. 1985 May;162(2):840-4 PMID: 3157679
  93. General nature of the genetic code for proteins.
    Nature. 1961 Dec 30;192:1227-32 PMID: 13882203
  94. Open reading frame expression vectors: a general method for antigen production in Escherichia coli using protein fusions to beta-galactosidase.
    Proc Natl Acad Sci U S A. 1983 Jul;80(14):4432-6 PMID: 6308625
  95. Protein secretion in Escherichia coli.
    Annu Rev Microbiol. 1985;39:615-48 PMID: 3904614
  96. Amino terminus of the yeast GAL4 gene product is sufficient for nuclear localization.
    Proc Natl Acad Sci U S A. 1984 Oct;81(19):5951-5 PMID: 6091123
  97. Lac repressor can be fused to beta-galactosidase.
    Nature. 1974 Jun 7;249(457):561-3 PMID: 4599764
  98. Synthetic multifunctional proteins: isolation of covalently linked tryptophan synthetase alpha-subunit-lac-repressor-beta-galactosidase chimeras.
    Mol Gen Genet. 1977 Oct 24;155(3):301-7 PMID: 414064
  99. Use of gene fusions and protein-protein interaction in the isolation of a biologically active regulatory protein: the replication initiator protein of plasmid R6K.
    Proc Natl Acad Sci U S A. 1983 Nov;80(22):6848-52 PMID: 6316329
  100. Heme regulates transcription of the CYC1 gene of S. cerevisiae via an upstream activation site.
    Cell. 1983 Apr;32(4):1279-86 PMID: 6301690
  101. Measurement of in vivo expression of the recA gene of Escherichia coli by using lacZ gene fusions.
    J Bacteriol. 1984 Oct;160(1):112-21 PMID: 6090405
  102. Export of protein in bacteria.
    Microbiol Rev. 1984 Dec;48(4):290-8 PMID: 6394976
  103. New ways to study developmental genes in spore-forming bacteria.
    Science. 1985 Apr 19;228(4697):285-91 PMID: 17790214
  104. Identification of OmpR: a positive regulatory protein controlling expression of the major outer membrane matrix porin proteins of Escherichia coli K-12.
    J Bacteriol. 1981 Jul;147(1):255-8 PMID: 6263866
  105. DNA-damaging agents stimulate gene expression at specific loci in Escherichia coli.
    Proc Natl Acad Sci U S A. 1980 May;77(5):2819-23 PMID: 6771759
  106. Growth-rate-dependent regulation of ribosome synthesis in E. coli: expression of the lacZ and galK genes fused to ribosomal promoters.
    Cell. 1981 Sep;25(3):773-82 PMID: 6793240
  107. Lactose genes fused to exogenous promoters in one step using a Mu-lac bacteriophage: in vivo probe for transcriptional control sequences.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4530-3 PMID: 159458
  108. Growth rate-dependent regulation of 6-phosphogluconate dehydrogenase level in Escherichia coli K-12: beta-galactosidase expression in gnd-lac operon fusion strains.
    J Bacteriol. 1983 Feb;153(2):771-81 PMID: 6337127
  109. Identification and characterization of a self-regulated repressor of translocation of the Tn3 element.
    Proc Natl Acad Sci U S A. 1979 Aug;76(8):4020-4 PMID: 386354
  110. Synthesis of cholera toxin is positively regulated at the transcriptional level by toxR.
    Proc Natl Acad Sci U S A. 1984 Jun;81(11):3471-5 PMID: 6374658
  111. Targeting of a foreign protein to chloroplasts by fusion to the transit peptide from the small subunit of ribulose 1,5-bisphosphate carboxylase.
    Nature. 1985 Jan 31-Feb 6;313(6001):358-63 PMID: 3969146
  112. Translational coupling during expression of the tryptophan operon of Escherichia coli.
    Genetics. 1980 Aug;95(4):785-95 PMID: 6162715
  113. A new insertion sequence, IS121, is found on the Mu dI1 (Ap lac) bacteriophage and the Escherichia coli K-12 chromosome.
    J Bacteriol. 1983 Nov;156(2):669-79 PMID: 6313612
  114. Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophage lambda and Mu.
    J Mol Biol. 1976 Jul 5;104(3):541-55 PMID: 781293
  115. Isolation and characterization of mutants of Escherichia coli K12 affected in protein localization.
    Methods Enzymol. 1983;97:11-40 PMID: 6228708
  116. A role for mRNA secondary structure in the control of translation initiation.
    Nature. 1982 Feb 18;295(5850):616-8 PMID: 6799842
  117. Physical characterization of ilv-lac fusions.
    J Bacteriol. 1979 Oct;140(1):251-60 PMID: 159287
  118. Purification, structure, and properties of hybrid beta-galactosidase proteins.
    J Biol Chem. 1983 Dec 10;258(23):14354-8 PMID: 6417139
  119. Rapid purification of a cloned gene product by genetic fusion and site-specific proteolysis.
    Proc Natl Acad Sci U S A. 1984 Aug;81(15):4692-6 PMID: 6087342
  120. E. coli mutant pleiotropically defective in the export of secreted proteins.
    Cell. 1981 Sep;25(3):765-72 PMID: 7026050
  121. Essential site for growth rate-dependent regulation within the Escherichia coli gnd structural gene.
    Proc Natl Acad Sci U S A. 1984 Dec;81(24):7669-73 PMID: 6440141
Article Info
Journal
Microbiological reviews
Abbr.
Microbiol Rev
ISSN
0146-0749
Published
1985-12-00
Pages
398-418
Language
English
Region
United States
NLM ID
7806086
PMCID
PMC373045
Subset
IM
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com