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PMID: 6787605 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Yeast genes fused to beta-galactosidase in Escherichia coli can be expressed normally in yeast.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 4 ·1981-04-00 ·Pages 2460-4

Rose M, Casadaban MJ, Botstein D

Abstract

A plasmid was constructed that allows the selection in vivo of gene fusions between the Escherichia coli beta-galactosidase gene and the yeast (Saccharomyces cerevisiae) URA3 gene. A large yeast DNA fragment containing the URA3 gene was placed upstream of an amino-terminally deleted version of the lacZ gene. The plasmid vehicle contains sequences that allow selection and maintenance of the plasmid in both yeast and E. coli. Selection for Lac+ in E. coli yielded numerous deletions that fused the lacZ gene to the URA3 gene and flanking yeast sequences, to the bacterial tetracycline-resistance gene from the parent plasmid pBR322, and to the yeast 2-micrometer plasmid DNA. Some of these fusion plasmids produced beta-galactosidase activity when introduced into yeast. One of the fusions to the URA3 gene itself has been shown to place the expression of beta-galactosidase activity under uracil regulation in yeasts.

MeSH Terms
Escherichia coli/genetics Galactosidases/genetics Gene Expression Regulation Genes, Regulator Genes, Synthetic Orotidine-5'-Phosphate Decarboxylase/genetics Plasmids Saccharomyces cerevisiae/genetics beta-Galactosidase/genetics
Chemicals
Galactosidases beta-Galactosidase Orotidine-5'-Phosphate Decarboxylase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Rose M
Casadaban M J
Botstein D
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30 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.78.4.2460
Published
1981-04-00
Pages
2460-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC319366
Subset
IM
Grants
NIGMS NIH HHS · GM18973 · United States
NIGMS NIH HHS · GM21253 · United States
External Links
PubMed source DOI: 10.1073/pnas.78.4.2460 PMC Full Text View journal details
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