Regulation of orotidylic acid pyrophosphorylase in Saccharomyces cerevisiae.

Journal of bacteriology ·Vol. 109 ·No. 1 ·1972-01-00 ·Pages 196-202

Jund R, Lacroute F

Abstract

Yeast mutants deficient in orotidine-5'-phosphate (OMP) pyrophosphorylase activity have been obtained. The gene encoding this enzyme is unlinked to the four pyrimidine genes previously described. The specific activity of OMP pyrophosphorylase remains constant under all of the physiological conditions used to repress, to derepress, or to induce pyrimidine biosynthesis. This enzyme appears, therefore, to escape the scheme of regulation by mixed repression and induction controlling the other enzymes of the pyrimidine pathway.

MeSH Terms

Amidohydrolases/metabolism Amino Acids/pharmacology Aspartic Acid/pharmacology Carbamates/pharmacology Carboxy-Lyases/metabolism Cell-Free System Culture Media Enzyme Induction Enzyme Repression Fluorouracil/pharmacology Genes Genetic Complementation Test Genetics, Microbial Mutation Orotic Acid/metabolism Oxidoreductases/metabolism Pentosyltransferases/metabolism Pyrimidines/biosynthesis Saccharomyces/enzymology Saccharomyces cerevisiae/enzymology,growth & development,metabolism Spectrophotometry Transferases/metabolism Uracil/analogs & derivatives,pharmacology Uracil Nucleotides/biosynthesis

Chemicals

Amino Acids Carbamates Culture Media Pyrimidines Uracil Nucleotides Aspartic Acid Uracil Orotic Acid Oxidoreductases Transferases Pentosyltransferases Amidohydrolases Carboxy-Lyases Fluorouracil

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Jund R

Lacroute F

References (12)

12 references, click to expand

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Studies on the control of pyrimidine biosynthesis in human diploid cell strains. II. Effects of 5-azaorotic acid, barbituric acid, and pyrimidine precursors on cellular phenotype.
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Regulation of pyrimidine biosynthesis in Saccharomyces cerevisiae.
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J Biol Chem. 1951 Sep;192(1):87-94 PMID: 14917654
Enzymatic synthesis of pyrimidine nucleotides; orotidine-5'-phosphate and uridine-5'-phosphate.
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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.109.1.196-202.1972

Published

1972-01-00

Pages

196-202

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC247267

Subset

External Links

PubMed source DOI: 10.1128/jb.109.1.196-202.1972 PMC Full Text View journal details

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Regulation of orotidylic acid pyrophosphorylase in Saccharomyces cerevisiae.

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