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PMID: 5361218 Published · ppublish English Journal Article

Pyrimidine synthesis in Neurospora crassa: gene-enzyme relationships.

Journal of bacteriology ·Vol. 100 ·No. 3 ·1969-12-00 ·Pages 1371-7

Caroline DF

Abstract

A new series of pyrimidine-requiring mutants of Neurospora has been isolated and all enzymes involved in pyrimidine biosynthesis are represented by at least one mutant. Among these mutants is included a single isolate for a new locus, pyr-6. This mutant is deficient in dihydroorotase (DHOase) and represents the only enzymatic step in orotate synthesis for which no mutant previously had been found. This mutant, which mapped genetically on the right arm of linkage group V, is unlinked to any of the other pyrimidine mutants. The DHOase-deficient mutant is also characterized by an unexpected growth behavior. The pyr-1 locus has been specifically associated with a lack of dihydroorotate dehydrogenase (DHOdehase). Mutants isolated in this series for other pyrimidine loci have been related to previously isolated mutants by allelism, recombination, and accumulation studies.

MeSH Terms
Alleles Amidohydrolases/metabolism Chromosome Mapping Genetic Complementation Test Genetics, Microbial Molecular Biology Mutation Neurospora/enzymology,metabolism Orotic Acid Oxidoreductases/metabolism Pyrimidines/biosynthesis Transferases/metabolism Uridine/metabolism
Chemicals
Pyrimidines Orotic Acid Oxidoreductases Transferases Amidohydrolases Uridine
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Caroline D F
References (21)
21 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.100.3.1371-1377.1969
Published
1969-12-00
Pages
1371-7
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC250342
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.100.3.1371-1377.1969 PMC Full Text View journal details
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