Home LiteratureArticle Details
PMID: 5361219 Published · ppublish English Journal Article

Pyrimidine synthesis in Neurospora crassa: regulation of enzyme activities.

Journal of bacteriology ·Vol. 100 ·No. 3 ·1969-12-00 ·Pages 1378-84

Caroline DF, Davis RH

Abstract

The regulation of several enzymes involved in pyrimidine biosynthesis in Neurospora crassa has been studied. Elevation of ATCase (l-aspartate carbamoyltransferase) activity is found in all pyrimidine-requiring mutants when they are starved for uridine. DHOase (dihydroorotase) is an unstable enzyme, and it is impossible to conclude what type of regulation, if any, controls this enzyme. DHOdehase (dihydroorotate dehydrogenase) activity shows a marked elevation in uridine-starved pyr-2 cultures, a mutant blocked late in the pathway. Several mutants blocked early in the pathway show much smaller increases in DHOdehase activity and possible explanations for this are discussed. Differences in the modes of regulation of the pyrimidine biosynthetic pathways in various organisms are compared.

MeSH Terms
Amidohydrolases/metabolism Enzyme Repression Mutation Neurospora/enzymology,metabolism Orotic Acid Oxidoreductases/metabolism Pyrimidines/biosynthesis Transferases/metabolism Uridine/metabolism
Chemicals
Pyrimidines Orotic Acid Oxidoreductases Transferases Amidohydrolases Uridine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Caroline D F
Davis R H
References (23)
23 references, click to expand
  1. Control by uracil of formation of enzymes required for orotate synthesis.
    J Biol Chem. 1957 Aug;227(2):677-92 PMID: 13462990
  2. Regulation of pyrimidine biosynthesis in Serratia marcescens.
    Proc Natl Acad Sci U S A. 1965 Jun;53(6):1483-9 PMID: 5324623
  3. Simultaneous Adaptation: A New Technique for the Study of Metabolic Pathways.
    J Bacteriol. 1947 Sep;54(3):339-48 PMID: 16561366
  4. Symptosium on multiple forms of enzymes and control mechanisms. II. Enzyme multiplicity and function in the regulation of divergent metabolic pathways.
    Bacteriol Rev. 1963 Jun;27:170-81 PMID: 13978658
  5. Translational control of gene expression.
    Cold Spring Harb Symp Quant Biol. 1966;31:321-33 PMID: 5237193
  6. Studies on the control of pyrimidine biosynthesis in human diploid cell strains. II. Effects of 5-azaorotic acid, barbituric acid, and pyrimidine precursors on cellular phenotype.
    Proc Natl Acad Sci U S A. 1967 May;57(5):1267-74 PMID: 5231733
  7. Regulation of pyrimidine biosynthesis in Saccharomyces cerevisiae.
    J Bacteriol. 1968 Mar;95(3):824-32 PMID: 5651325
  8. Dihydroorotic acid dehydrogenase activity of human diploid cell strains.
    Science. 1968 May 3;160(3827):539-41 PMID: 5644059
  9. Control of pyrimidine biosynthesis in Pseudomonas aeruginosa.
    J Bacteriol. 1968 Nov;96(5):1732-41 PMID: 4973129
  10. [PHYSIOLOGICAL STUDY OF MUTANTS RESISTANT TO 5-FLUOROURACIL IN YEAST].
    C R Hebd Seances Acad Sci. 1964 Feb 17;258:2172-4 PMID: 14139968
  11. Evolutionary significance of metabolic control systems. The beta-ketoadipate pathway provides a case history in bacteria.
    Science. 1967 Jun 30;156(3783):1695-9 PMID: 5611030
  12. Studies on the control of pyrimidine biosynthesis in human diploid cell strains, I. Effect of 6-azauridine on cellular phenotype.
    Proc Natl Acad Sci U S A. 1967 Apr;57(4):925-32 PMID: 5231355
  13. [Inducible enzymatic systems of the metabolism of carbohydrates by Escherichia coli].
    Adv Enzymol Relat Areas Mol Biol. 1968;30:81-137 PMID: 4872301
  14. Carbamyl phosphate synthesis in Neurospora crassa. II. Genetics, metabolic position, and regulation of arginine-specific carbamyl phosphokinase.
    Biochim Biophys Acta. 1965 Aug 24;107(1):54-68 PMID: 5857368
  15. Coordination of the synthesis of the enzymes in the pyrimidine pathway of E. coli.
    J Mol Biol. 1962 Dec;5:618-34 PMID: 13970180
  16. Pyrimidine synthesis in Neurospora crassa: gene-enzyme relationships.
    J Bacteriol. 1969 Dec;100(3):1371-7 PMID: 5361218
  17. The relationship between gene suppression and aspartate transcarbamylase activity in pyr-3 mutants of Neurospora.
    Genetics. 1962 Aug;47:1075-83 PMID: 14025516
  18. Intracellular Regulatory Mechanisms: Regulation in multicellular forms may be an elaboration upon the pattern evolved in microorganisms.
    Science. 1964 Aug 14;145(3633):674-9 PMID: 17754663
  19. BIOCHEMICAL ASPECTS OF GENETICS.
    Annu Rev Biochem. 1965;34:527-64 PMID: 14321179
  20. Studies on the roles of synthesis and degradation in the control of enzyme levels in animal tissues.
    Bull Soc Chim Biol (Paris). 1966;48(10):1009-30 PMID: 4959571
  21. THE REGULATION OF PYRIMIDINE BIOSYNTHESIS IN NEUROSPORA CRASSA. I. END-PRODUCT INHIBITION AND REPRESSION OF ASPARTATE CARBAMOYLTRANSFERASE.
    Biochim Biophys Acta. 1964 Feb 10;82:284-92 PMID: 14123563
  22. The regulation of synthesis of leucine biosynthetic enzymes in Neurospora.
    Proc Natl Acad Sci U S A. 1965 Dec;54(6):1538-46 PMID: 5218909
  23. REGULATORY MECHANISMS GOVERNING SYNTHESIS OF THE ENZYMES FOR TRYPTOPHAN OXIDATION BY PSEUDOMONAS FLUORESCENS.
    J Gen Microbiol. 1964 May;35:319-34 PMID: 14179678
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.100.3.1378-1384.1969
Published
1969-12-00
Pages
1378-84
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC250345
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.100.3.1378-1384.1969 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2