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PMID: 6450745 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Deletion map of the Escherichia coli structural gene for alkaline phosphatase, phoA.

Journal of bacteriology ·Vol. 145 ·No. 1 ·1981-01-00 ·Pages 288-92

Sarthy A, Michaelis S, Beckwith J

Abstract

Lambda transducing phages containing portions of the phoA gene have been isolated and used to construct a deletion map of the phoA gene. The isolation of a plaque-forming lambda transducing phage carrying the entire phoA gene is also described. Two new methods for screening or selection of mutants that have altered levels of alkaline phosphatase activity are reported.

MeSH Terms
Alkaline Phosphatase/genetics Bacteriophage lambda/genetics Chromosome Mapping Chromosomes, Bacterial Escherichia coli/enzymology,genetics Genes Mutation Recombination, Genetic Transduction, Genetic
Chemicals
Alkaline Phosphatase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Sarthy A
Michaelis S
Beckwith J
References (11)
11 references, click to expand
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  7. Use of gene fusions to determine a partial signal sequence of alkaline phosphatase.
    J Bacteriol. 1979 Sep;139(3):932-9 PMID: 113391
  8. Structure of the malB region in Escherichia coli K12. II. Genetic map of the malE,F,G operon.
    Mol Gen Genet. 1979 Jul 24;174(3):249-59 PMID: 384167
  9. Escherichia coli pleiotropic mutant that reduces amounts of several periplasmic and outer membrane proteins.
    J Bacteriol. 1979 Oct;140(1):229-39 PMID: 387722
  10. Linkage map of Escherichia coli K-12, edition 6.
    Microbiol Rev. 1980 Mar;44(1):1-56 PMID: 6997720
  11. Use of gene fusions to determine the orientation of gene phoA on the Escherichia coli chromosome.
    J Bacteriol. 1981 Jan;145(1):293-8 PMID: 7007316
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.145.1.288-292.1981
Published
1981-01-00
Pages
288-92
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC217271
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.145.1.288-292.1981 PMC Full Text View journal details
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