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PMID: 7007316 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Use of gene fusions to determine the orientation of gene phoA on the Escherichia coli chromosome.

Journal of bacteriology ·Vol. 145 ·No. 1 ·1981-01-00 ·Pages 293-8

Sarthy A, Michaelis S, Beckwith J

Abstract

We present genetic evidence which demonstrates that the phoA gene is transcribed in the clockwise direction on the Escherichia coli chromosome, in contrast to an earlier proposal. Our conclusion is based on analysis of various genetic fusions between the lac operon and the phoA gene.

MeSH Terms

Alkaline Phosphatase/genetics Chromosomes, Bacterial DNA, Recombinant Escherichia coli/enzymology,genetics Genes Lac Operon Mutation Transcription, Genetic

Chemicals

DNA, Recombinant Alkaline Phosphatase

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Sarthy A

Michaelis S

Beckwith J

References (11)

11 references, click to expand

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J Mol Biol. 1969 Nov 14;45(3):549-66 PMID: 4917369
Further genetic mapping of the phoA-phoR region for alkaline phosphatase synthesis in Escherichia coli K 12.
Mol Gen Genet. 1970;109(1):18-26 PMID: 4922680
Location and orientation of the phoA locus on the Escherichia coli K-12 linkage map.
J Bacteriol. 1971 Sep;107(3):683-9 PMID: 4937782
Use of gene fusion to study secretion of maltose-binding protein into Escherichia coli periplasm.
J Bacteriol. 1979 Jul;139(1):19-31 PMID: 110778
Nonsense mutants and polarity in the lac operon of Escherichia coli.
J Mol Biol. 1965 Nov;14(1):290-6 PMID: 5327654
Use of gene fusions to determine a partial signal sequence of alkaline phosphatase.
J Bacteriol. 1979 Sep;139(3):932-9 PMID: 113391
The use of gene fusions to study the expression of malT the positive regulator gene of the maltose regulon.
J Mol Biol. 1979 Aug 15;132(3):521-34 PMID: 118263
Linkage map of Escherichia coli K-12, edition 6.
Microbiol Rev. 1980 Mar;44(1):1-56 PMID: 6997720
Deletion map of the Escherichia coli structural gene for alkaline phosphatase, phoA.
J Bacteriol. 1981 Jan;145(1):288-92 PMID: 6450745
Influence of inorganic phosphate in the formation of phosphatases by Escherichia coli.
Biochim Biophys Acta. 1960 Mar 11;38:460-9 PMID: 13838951
Structure of the malB region in Escherichia coli K12. II. Genetic map of the malE,F,G operon.
Mol Gen Genet. 1979 Jul 24;174(3):249-59 PMID: 384167

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.145.1.293-298.1981

Published

1981-01-00

Pages

293-8

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC217272

Subset

External Links

PubMed source DOI: 10.1128/jb.145.1.293-298.1981 PMC Full Text View journal details

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Use of gene fusions to determine the orientation of gene phoA on the Escherichia coli chromosome.

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