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PMID: 16559103 Published · ppublish English Journal Article

Fusions of the lac and trp Regions of the Escherichia coli Chromosome.

Journal of bacteriology ·Vol. 104 ·No. 3 ·1970-12-00 ·Pages 1273-9

Miller JH, Reznikoff WS, Silverstone AE, Ippen K, Signer ER, Beckwith JR

Abstract

Two classes of strains were studied in which the lac operon is transposed to a chromosomal site close to the tonB and trp loci. The two classes differ in the orientation of the lac region on the chromosome. In both types of strains, tonB mutants were selected in which deletions removing the tonB locus also caused a fusion of the lac and trp regions. The study of the properties of such fusion strains provides information on the control of both the lac and trp operons.

Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Miller J H
Department of Bacteriology and Immunology, Harvard Medical School, Boston, Massachusetts 02115.
Reznikoff W S
Silverstone A E
Ippen K
Signer E R
Beckwith J R
References (25)
25 references, click to expand
  1. Revised linkage map of Escherichia coli.
    Bacteriol Rev. 1967 Dec;31(4):332-53 PMID: 4865540
  2. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  3. Polarity in the tryptophan operon of E. coli.
    Cold Spring Harb Symp Quant Biol. 1966;31:235-49 PMID: 4866380
  4. Transposition of the Lac region of E. coli.
    Cold Spring Harb Symp Quant Biol. 1966;31:393-401 PMID: 4866389
  5. New controlling element in the Lac operon of E. coli.
    Nature. 1968 Mar 2;217(5131):825-7 PMID: 4867974
  6. Direction of transcription of a regulatory gene in E. coli.
    Nature. 1968 Dec 28;220(5174):1287-90 PMID: 4882916
  7. The non-linear relationship between the enzyme activity and structural protein concentration of thiogalactoside transacetylase of E. coli.
    Biochem Biophys Res Commun. 1969 Feb 21;34(4):522-7 PMID: 4887461
  8. The promoter-operator region of the lac operon of Escherichia coli.
    J Mol Biol. 1968 Dec;38(3):413-20 PMID: 4887877
  9. Direction of in vivo degradation of tryptophan messenger RNA--a correction.
    Nature. 1969 Jul 5;223(5201):40-3 PMID: 4893261
  10. Consequences of deletion mutations joining two operons of opposite polarity.
    J Mol Biol. 1969 Jun 28;42(3):511-20 PMID: 4896188
  11. A mechanism for repressor action.
    J Mol Biol. 1969 Jul 14;43(1):201-13 PMID: 4897791
  12. Genetic evidence that the operator locus is distinct from the the z gene in the lac operon of Escherichia coli.
    J Mol Biol. 1969 Jul 14;43(1):215-8 PMID: 4897792
  13. Biochemical evidence that the operator locus is distinct from the z gene in the lac operon of Escherichia coli.
    J Mol Biol. 1969 Jul 14;43(1):219-22 PMID: 4897793
  14. Directed transposition of the arabinose operon: a technique for the isolation of specialized transducing bacteriophages for any Escherichia coli gene.
    J Mol Biol. 1969 Aug 28;44(1):117-27 PMID: 4897798
  15. Regulation of the enzymes of the tryptophan pathway in Escherichia coli.
    Genetics. 1965 Dec;52(6):1303-16 PMID: 5327408
  16. Mapping of suppressor loci in Escherichia coli.
    J Mol Biol. 1965 Nov;14(1):153-66 PMID: 5327649
  17. Nonsense mutants and polarity in the lac operon of Escherichia coli.
    J Mol Biol. 1965 Nov;14(1):290-6 PMID: 5327654
  18. Interaction of prophages at the att80 site with the chromosome of Escherichia coli.
    J Mol Biol. 1966 Jan;15(1):243-55 PMID: 5330218
  19. The functional organization of the tryptophan gene cluster in Salmonella typhimurium.
    Proc Natl Acad Sci U S A. 1966 Jul;56(1):111-8 PMID: 5338585
  20. Transposition of the lac region of Escherichia coli. I. Inversion of the lac operon and transduction of lac by phi80.
    J Mol Biol. 1966 Aug;19(2):254-65 PMID: 5338856
  21. Nonsense codons and polarity in the tryptophan operon.
    J Mol Biol. 1966 Nov 14;21(2):313-34 PMID: 5339605
  22. Chorismic acid: purification and some chemical and physical studies.
    Biochem J. 1964 Feb;90(2):256-61 PMID: 5834235
  23. A DELETION ANALYSIS OF THE LAC OPERATOR REGION IN ESCHERICHIA COLI.
    J Mol Biol. 1964 Mar;8:427-30 PMID: 14168696
  24. A SECOND PERMEASE FOR METHYL-THIO-BETA-D-GALACTOSIDE IN ESCHERICHIA COLI.
    Biochim Biophys Acta. 1965 May 4;100:591-3 PMID: 14347955
  25. Frameshift mutations in the lactose operon of E. coli.
    Cold Spring Harb Symp Quant Biol. 1966;31:189-201 PMID: 4866375
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.104.3.1273-1279.1970
Published
1970-12-00
Pages
1273-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC248287
External Links
PubMed source DOI: 10.1128/jb.104.3.1273-1279.1970 PMC Full Text View journal details
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