Home LiteratureArticle Details
PMID: 97515 Published · ppublish English Journal Article

New regulatory mutations affecting the expression of the threonine operon in Escherichia coli K-12.

Molecular & general genetics : MGG ·Vol. 162 ·No. 1 ·1978-06-01 ·Pages 95-100

Saint-Girons I

Abstract

The promoter of the threonine operon was joined to the structural genes of the lac operon in Escherichia coli K 12. The synthesis of beta-galactosidase was thus repressed by threonine plus isoleucine in the fusion strains. To isolate mutations which affect the expression of the threonine operon, alterations in the level of expression of the lacZ gene were selected. A new type of regulatory mutation was discovered.

MeSH Terms
Escherichia coli/genetics,isolation & purification Galactosidases/genetics Genes Genes, Regulator Lactose/genetics Mutation Operon Threonine/genetics Transduction, Genetic beta-Galactosidase/genetics
Chemicals
Threonine Galactosidases beta-Galactosidase Lactose
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Saint-Girons I
References (14)
14 references, click to expand
  1. Bacteriophage Mu integration: on the mechanism of Mu-induced mutations.
    J Mol Biol. 1971 Nov 28;62(1):171-8 PMID: 4945527
  2. Threonine locus of Escherichia coli K-12: genetic structure and evidence for an operon.
    J Bacteriol. 1974 Jun;118(3):990-8 PMID: 4364333
  3. Operator-promoter functions in the threonine operon of Escherichia coli.
    J Bacteriol. 1975 Oct;124(1):161-6 PMID: 170244
  4. Prophage lambda at unusual chromosomal locations. II. Mutations induced by bacteriophage lambda in Escherichia coli K12.
    J Mol Biol. 1973 Oct 25;80(2):297-314 PMID: 4587404
  5. Revised structure of aspartokinase I-homoserine dehydrogenase I of Escherichia coli K12. Evidence for four identical subunits.
    Eur J Biochem. 1972 Aug 4;28(4):507-19 PMID: 4562989
  6. Operator-constitutive mutants in the threonine operon of Escherichia coli K-12.
    J Bacteriol. 1975 Dec;124(3):1137-41 PMID: 172487
  7. Determination of serum proteins by means of the biuret reaction.
    J Biol Chem. 1949 Feb;177(2):751-66 PMID: 18110453
  8. Regulation of the regulatory gene for the arabinose pathway, araC.
    J Mol Biol. 1976 Jul 5;104(3):557-66 PMID: 781294
  9. A simple method for making new transducing lines of coliphage lambda.
    Mol Gen Genet. 1975;137(2):101-7 PMID: 1186657
  10. Isoleucine and valine metabolism of Escherichia coli. XIV. Effect of thiaisoleucine.
    J Bacteriol. 1968 May;95(5):1666-71 PMID: 4870281
  11. Threonyl-transfer ribonucleic acid synthetase and the regulation of the threonine operon in Escherichia coli.
    J Bacteriol. 1977 Jan;129(1):66-70 PMID: 318654
  12. Conversion of beta-galactosidase to a membrane-bound state by gene fusion.
    Proc Natl Acad Sci U S A. 1976 Oct;73(10):3423-7 PMID: 790385
  13. Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophage lambda and Mu.
    J Mol Biol. 1976 Jul 5;104(3):541-55 PMID: 781293
  14. Multivalent repression in the biosynthesis of threonine in Salmonella typhimurium and Escherichia coli.
    Biochem Biophys Res Commun. 1963 Feb 6;10:277-82 PMID: 13959617
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00333855
Published
1978-06-01
Pages
95-100
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00333855
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2