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PMID: 8391110 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The VirD2 protein of Agrobacterium tumefaciens carries nuclear localization signals important for transfer of T-DNA to plant.

Molecular & general genetics : MGG ·Vol. 239 ·No. 3 ·1993-06-00 ·Pages 345-53

Rossi L, Hohn B, Tinland B

Abstract

Agrobacterium tumefaciens is able to transfer a piece of DNA, the T-DNA, to the nucleus of the plant cell. The VirD2 protein is required for the production of the T-DNA, it is tightly linked to the T-DNA and it is thought to direct it to the plant genome. Two nuclear localization signals (NLS), one in the N-terminal part and one in the C-terminal part of the VirD2 protein, have been shown to be able to target marker proteins to the plant nucleus. Here we analyze nuclear entry of the T-DNA complex using a new and very sensitive assay for T-DNA transfer. We show that optimal T-DNA transfer requires the VirD2 NLS located in the C-terminal part of the protein, whereas mutations in the N-terminal NLS coding sequence seem to have no effect on T-DNA transfer.

Related Genes

uidA virD2

MeSH Terms

Agrobacterium tumefaciens/genetics,pathogenicity Amino Acid Sequence Bacterial Proteins/genetics Base Sequence Cell Nucleus/metabolism DNA Transposable Elements DNA, Bacterial/genetics Gene Deletion Gene Expression Regulation, Neoplastic Genes, Plant Molecular Sequence Data Mutagenesis, Insertional Oncogenes Plant Tumors/microbiology Plants, Toxic Plasmids Protein Sorting Signals/genetics,metabolism Tobacco/genetics Transfection Transformation, Genetic Virulence/genetics Virulence Factors beta-Galactosidase/metabolism

Chemicals

Bacterial Proteins DNA Transposable Elements DNA, Bacterial Protein Sorting Signals Virulence Factors beta-Galactosidase

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Rossi L

Friedrich Miescher-Institut, Basel, Switzerland.

Hohn B

Tinland B

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34 references, click to expand

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Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00276932

Published

1993-06-00

Pages

345-53

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00276932

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The VirD2 protein of Agrobacterium tumefaciens carries nuclear localization signals important for transfer of T-DNA to plant.

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