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PMID: 7621833 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Trans-kingdom T-DNA transfer from Agrobacterium tumefaciens to Saccharomyces cerevisiae.

The EMBO journal ·Vol. 14 ·No. 13 ·1995-07-03 ·Pages 3206-14

Bundock P, den Dulk-Ras A, Beijersbergen A, Hooykaas PJ

Abstract

Agrobacterium tumefaciens transfers part of its tumour-inducing (Ti) plasmid, the transferred or T-DNA, to plants during tumourigenesis. This represents the only example of naturally occurring trans-kingdom transfer of genetic material. Here we report that A.tumefaciens can transfer its T-DNA not only to plant cells, but also to another eukaryote, namely the yeast Saccharomyces cerevisiae. The Ti plasmid virulence (vir) genes that mediate T-DNA transfer to plants were found to be essential for transfer to yeast as well. Transgenic S.cerevisiae strains were analysed for their T-DNA content. Results showed that T-DNA circles were formed in yeast with precise fusions between the left and right borders. Such T-DNA circles were stably maintained by the yeast if the replicator from the yeast 2 mu plasmid was present in the T-DNA. Integration of T-DNA in the S.cerevisiae genome was found to occur via homologous recombination. This contrasts with integration in the plant genome, where T-DNA integrates preferentially via illegitimate recombination. Our results thus suggest that the process of T-DNA integration is predominantly determined by host factors.

MeSH Terms
Bacterial Proteins/genetics Base Sequence DNA, Bacterial Endodeoxyribonucleases/genetics Gene Expression Regulation, Fungal Genes, Bacterial Genes, Fungal Molecular Sequence Data Plasmids Recombination, Genetic Rhizobium/genetics,pathogenicity Saccharomyces cerevisiae/genetics Transcription Factors/genetics Transformation, Bacterial Virulence Virulence Factors
Chemicals
Bacterial Proteins DNA, Bacterial T-DNA Transcription Factors Virulence Factors Endodeoxyribonucleases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Bundock P
Institute of Molecular Plant Sciences, Clusius Laboratory, Leiden University, The Netherlands.
den Dulk-Ras A
Beijersbergen A
Hooykaas P J
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1995-07-03
Pages
3206-14
Language
English
Region
England
NLM ID
8208664
PMCID
PMC394382
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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