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PMID: 3889831 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Completion of the nucleotide sequence of the central region of Tn5 confirms the presence of three resistance genes.

Nucleic acids research ·Vol. 13 ·No. 1 ·1985-01-11 ·Pages 195-205

Mazodier P, Cossart P, Giraud E, Gasser F

Abstract

The DNA sequence of the region located downstream from the kanamycin resistance gene of Tn5 up to the right inverted repeat IS50R has been determined. This completes the determination of the sequence of Tn5 which is 5818 bp long. The 2.7 Kb central region contains three resistance genes: the kanamycin-neomycin resistance gene, a gene coding for resistance to CL990 an antimitotic-antibiotic compound of the bleomycin family and a third gene that confers streptomycin resistance in some bacterial species but is cryptic in E. coli. A Tn5* mutant able to express streptomycin resistance in E. coli was isolated. With this mutant, it was demonstrated that in E. coli the expression of the three resistance genes is coordinated in a single operon.

MeSH Terms

Amino Acid Sequence Bacterial Proteins/genetics Base Sequence DNA, Bacterial/genetics Drug Resistance, Microbial Escherichia coli/drug effects,genetics Genes, Bacterial Kanamycin/pharmacology R Factors Streptomycin/pharmacology

Chemicals

Bacterial Proteins DNA, Bacterial Kanamycin Streptomycin

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Mazodier P

Cossart P

Giraud E

Gasser F

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27 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/13.1.195

Published

1985-01-11

Pages

195-205

Language

English

Region

England

NLM ID

0411011

PMCID

PMC340984

Subset

Databases

GENBANK

X01702

External Links

PubMed source DOI: 10.1093/nar/13.1.195 PMC Full Text View journal details

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Completion of the nucleotide sequence of the central region of Tn5 confirms the presence of three resistance genes.

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