Home LiteratureArticle Details
PMID: 6310563 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Nucleotide sequence of a streptomycete aminoglycoside phosphotransferase gene and its relationship to phosphotransferases encoded by resistance plasmids.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 80 ·No. 17 ·1983-09-00 ·Pages 5190-4

Thompson CJ, Gray GS

Abstract

The DNA sequence of an aminoglycoside phosphotransferase gene (aph) from Streptomyces fradiae ATCC 10745 (a neomycin producer) was determined. The gene was localized by in vitro subcloning and insertional inactivation. Molecular weight, amino acid composition, and amino-terminal analysis of the purified aph gene product confirmed the accuracy and position of the aph gene sequence. Pairwise comparisons of S. fradiae aph with the aph genes encoded by bacterial transposons Tn5 and Tn903 showed significant nucleotide and amino acid homologies which indicated a common evolutionary origin for these antibiotic-resistance genes.

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular Codon DNA Restriction Enzymes Drug Resistance, Microbial Genes Genes, Bacterial Kanamycin Kinase Neomycin/pharmacology Phosphotransferases/genetics Plasmids Streptomyces/drug effects,enzymology,genetics
Chemicals
Codon Phosphotransferases Kanamycin Kinase DNA Restriction Enzymes Neomycin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Thompson C J
Gray G S
References (25)
25 references, click to expand
  1. Aminoglycoside antibiotic-inactivating enzymes in actinomycetes similar to those present in clinical isolates of antibiotic-resistant bacteria.
    Proc Natl Acad Sci U S A. 1973 Aug;70(8):2276-80 PMID: 4209515
  2. Estimation of secondary structure in ribonucleic acids.
    Nature. 1971 Apr 9;230(5293):362-7 PMID: 4927725
  3. Control of transcription termination.
    Annu Rev Biochem. 1978;47:967-96 PMID: 354508
  4. Plasmid-determined resistance to antimicrobial agents.
    Annu Rev Microbiol. 1978;32:469-518 PMID: 360974
  5. Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells.
    Gene. 1979 May;6(1):23-8 PMID: 383576
  6. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
    Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PMID: 388356
  7. Regulatory sequences involved in the promotion and termination of RNA transcription.
    Annu Rev Genet. 1979;13:319-53 PMID: 94251
  8. A DNA cloning system for interspecies gene transfer in antibiotic-producing Streptomyces.
    Nature. 1980 Apr 10;284(5756):526-31 PMID: 7366721
  9. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  10. DNA cloning in Streptomyces: resistance genes from antibiotic-producing species.
    Nature. 1980 Jul 31;286(5772):525-7 PMID: 6250070
  11. Codon catalog usage is a genome strategy modulated for gene expressivity.
    Nucleic Acids Res. 1981 Jan 10;9(1):r43-74 PMID: 7208352
  12. Cloning and expression in streptomyces lividans of antibiotic resistance genes derived from Escherichia coli.
    J Bacteriol. 1981 Apr;146(1):360-8 PMID: 7012122
  13. Nucleotide sequence of the kanamycin resistance transposon Tn903.
    J Mol Biol. 1981 Apr 5;147(2):217-26 PMID: 6270337
  14. Translational initiation in prokaryotes.
    Annu Rev Microbiol. 1981;35:365-403 PMID: 6170248
  15. Nucleotide sequences of two Bacillus subtilis promoters used by Bacillus subtilis sigma-28 RNA polymerase.
    Nucleic Acids Res. 1981 Nov 25;9(22):5991-6000 PMID: 6273817
  16. Gene cloning in Streptomyces.
    Curr Top Microbiol Immunol. 1982;96:69-95 PMID: 6276096
  17. pIJ101, a multi-copy broad host-range Streptomyces plasmid: functional analysis and development of DNA cloning vectors.
    Mol Gen Genet. 1982;185(2):223-8 PMID: 6283316
  18. Cloning of antibiotic resistance and nutritional genes in streptomycetes.
    J Bacteriol. 1982 Aug;151(2):668-77 PMID: 6284706
  19. Biochemical characterization of resistance determinants cloned from antibiotic-producing streptomycetes.
    J Bacteriol. 1982 Aug;151(2):678-85 PMID: 6284707
  20. Gene expression in Streptomyces: construction and application of promoter-probe plasmid vectors in Streptomyces lividans.
    Mol Gen Genet. 1982;187(2):265-77 PMID: 6294463
  21. Nucleotide sequence and exact localization of the neomycin phosphotransferase gene from transposon Tn5.
    Gene. 1982 Oct;19(3):327-36 PMID: 6295884
  22. Physical analysis of antibiotic-resistance genes from Streptomyces and their use in vector construction.
    Gene. 1982 Nov;20(1):51-62 PMID: 6298066
  23. Expression of a neomycin phosphotransferase gene from Streptomyces fradiae in Escherichia coli after interplasmidic recombination.
    Mol Gen Genet. 1983;189(1):27-33 PMID: 6304462
  24. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  25. Lambdoid phages that simplify the recovery of in vitro recombinants.
    Mol Gen Genet. 1977 Jan 7;150(1):53-61 PMID: 319344
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.80.17.5190
Published
1983-09-00
Pages
5190-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC384217
Subset
IM
Databases
GENBANK
K00432
External Links
PubMed source DOI: 10.1073/pnas.80.17.5190 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2