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PMID: 6273817 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Nucleotide sequences of two Bacillus subtilis promoters used by Bacillus subtilis sigma-28 RNA polymerase.

Nucleic acids research ·Vol. 9 ·No. 22 ·1981-11-25 ·Pages 5991-6000

Gilman MZ, Wiggs JL, Chamberlin MJ

Abstract

RNA polymerase holoenzymes from many bacterial species share a common promoter recognition specificity since they use the same promoter sites on a variety of templates. These promoters generally include sequences homologous to the average sequences -TTGACA- and -TATAATA-, located -35 and -10 base pairs, respectively, upstream of the transcriptional state site. We have isolated a minor form of B. subtilis RNA polymerase in which the normal sigma subunit (sigma 55) is replaced by a smaller polypeptide (sigma 28) and which is highly specific for a class of promoter sites not used by the sigma 55-holoenzyme. Sequencing of two B. subtilis promoter sites used by the sigma 28-holoenzyme reveals identical sequences at -35 and -10 base pairs from the start site, which are -CTAAA- and -CCGATAT-, respectively. These results confirm that sigma subunit plays a major direct role in promoter sequence recognition, and support a model in which sigma interacts sequentially with -35 and -10 regions, respectively.

MeSH Terms
Bacillus subtilis/enzymology,genetics Base Composition Base Sequence Cloning, Molecular DNA Restriction Enzymes DNA-Directed RNA Polymerases/metabolism Operon Plasmids Sigma Factor/genetics Transcription Factors/genetics Transcription, Genetic
Chemicals
Sigma Factor Transcription Factors DNA-Directed RNA Polymerases DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Gilman M Z
Wiggs J L
Chamberlin M J
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30 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/9.22.5991
Published
1981-11-25
Pages
5991-6000
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327579
Subset
IM
Grants
NIGMS NIH HHS · GM 07232 · United States
NIGMS NIH HHS · GM 12010 · United States
External Links
PubMed source DOI: 10.1093/nar/9.22.5991 PMC Full Text View journal details
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