Plasmid detection and sizing in single colony lysates.

Science (New York, N.Y.) ·Vol. 195 ·No. 4276 ·1977-01-28 ·Pages 393-4

Barnes WM

Abstract

A simple and contained procedure for the rapid assay of the presence and size of plasmids similar to Col El is described. Bacteria are picked from an agar plate with a toothpick, lysed with dodecyl sulfate and heat, and placed directly on an agarose gel for electrophoresis.

MeSH Terms

Colicins DNA, Bacterial/analysis DNA, Circular/analysis Electrophoresis, Agar Gel Escherichia coli Extrachromosomal Inheritance Methods Nucleic Acid Conformation Plasmids

Chemicals

Colicins DNA, Bacterial DNA, Circular

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Barnes W M

Article Info

Journal

Science (New York, N.Y.)

Abbr.

Science

ISSN

0036-8075

DOI

10.1126/science.318764

Published

1977-01-28

Pages

393-4

Language

English

Region

United States

NLM ID

0404511

Subset

External Links

PubMed source DOI: 10.1126/science.318764 View journal details

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