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PMID: 6777632 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Nucleotide sequence of a promoter recognized by Bacillus subtilis RNA polymerase.

Molecular & general genetics : MGG ·Vol. 180 ·No. 1 ·1980-00-00 ·Pages 57-65

Lee G, Talkington C, Pero J

Abstract

We report the nucleotide sequence of a promoter recognized by RNA polymerase from the gram-positive bacterium Bacillus subtilis. This promoter, which was isolated from B. subtilis phage SP01 DNA, is homologous to promoters for Escherichia coli RNA polymerase; the sequences of the "-35 region" and the "Pribnow box" were 5'TTGACT and 5'CATAAT, respectively (T is the thymine analog 5-hydroxymethyluracil in SP01 DNA). These sequences each differed by only a single base pair from the preferred sequences for E. coli promoters. Not surprisingly, the SP01 promoter was actively transcribed in vitro by E. coli RNA Polymerase as well as by B. subtilis RNA polymerase.

MeSH Terms
Bacillus subtilis/enzymology,genetics Bacteriophages/enzymology Base Sequence DNA, Bacterial/analysis DNA-Directed RNA Polymerases/genetics,metabolism Escherichia coli/enzymology,genetics Operon Substrate Specificity
Chemicals
DNA, Bacterial DNA-Directed RNA Polymerases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Lee G
Talkington C
Pero J
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23 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00267352
Published
1980-00-00
Pages
57-65
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00267352
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