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PMID: 319344 Published · ppublish English Journal Article

Lambdoid phages that simplify the recovery of in vitro recombinants.

Molecular & general genetics : MGG ·Vol. 150 ·No. 1 ·1977-01-07 ·Pages 53-61

Murray NE, Brammar WJ, Murray K

Abstract

Derivatives of phage lambda are described for use as vectors for fragments of DNA generated with the HindIII and EcoRI restriction enzymes. With some vectors, hybrid molecules are recognised by a change from a turbid to a clear plaque morphology resulting from the insertion of a fragment of DNA into the lambda gene coding for the phage regressor. Other vectors contain a central, replaceable fragment of DNA which imparts a readily recognisable phenotype. This central fragment may include either a gene for a mutant transfer RNA (suppressor) or a part of the lacZ gene of E. coli able to complement a lacZ host. The appropriate lacZ host and indicator plates permit the ready distinction between recombinant and vector phages by the colour of the plaques.

MeSH Terms
Coliphages DNA, Recombinant DNA, Viral Escherichia coli Genes Genetic Complementation Test Recombination, Genetic Viral Plaque Assay
Chemicals
DNA, Recombinant DNA, Viral
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Murray N E
Brammar W J
Murray K
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52 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF02425325
Published
1977-01-07
Pages
53-61
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF02425325
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