Bacteriophage lambda having EcoRI endonuclease sites only in the nonessential region of the genome.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 71 ·No. 10 ·1974-10-00 ·Pages 3927-30

Rambach A, Tiollais P

Abstract

A derivative of lambda b221 that has lost by mutation all EcoRI restriction sites has been isolated by alternative growth on restrictive and nonrestrictive strains. It has an efficiency of plating equal to 1 on the restrictive strain. Genetic cross of this bacteriophage with lambda plac5 imm21 gave rise to recombinants of intermediate restricting ratios. The analysis of the EcoRI endonuclease-cleaved DNA by polyacrylamide gel electrophoresis, compared with the genetic results, has permitted identification of EcoRI endonuclease cleavage sites in the recombinants. The genotypes are: lambda plac5 CI857 sRIlambda3(0)sRIlambda2(0)sRIlambda1(0) and lambda plac5 CI857 sRIlambda2(0)sRIlambda1(0). The remaining cleavage sites, respectively, sRIlac sRIlambda4 and sRIlac sRIlambda4 sRIlambda3, are all located in a region nonessential for bacteriophage multiplication. The involvement of these mutant bacteriophages as vector for foreign genes are discussed.

MeSH Terms

Binding Sites Chromosome Mapping Coliphages/analysis,isolation & purification Crosses, Genetic DNA, Viral/metabolism Electrophoresis, Polyacrylamide Gel Endonucleases/metabolism Genes Hydrolysis Mutation Polynucleotides/analysis Recombination, Genetic

Chemicals

DNA, Viral Polynucleotides Endonucleases

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Rambach A

Tiollais P

References (11)

11 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.71.10.3927

Published

1974-10-00

Pages

3927-30

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC434299

Subset

External Links

PubMed source DOI: 10.1073/pnas.71.10.3927 PMC Full Text View journal details

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Bacteriophage lambda having EcoRI endonuclease sites only in the nonessential region of the genome.

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