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PMID: 1083914 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Location in bacteriophage lamdba DNA of cleavage sites of the site-specific endonuclease from Bacillus amyloliquefaciens H.

Journal of virology ·Vol. 18 ·No. 2 ·1976-05-00 ·Pages 659-63

Haggerty DM, Scheif RF

Abstract

The sites in Escherichia coli bacteriophage lambda DNA cleaved by the site-specific endonuclease isolated from Bacillus amyloliquefaciens H (BamI) are found to be at 0.114, 0.466, 0.580, 0.713, and 0.861 lambda units. The sites were located by analysis of the products of digestion of lambda DNA and lambda-ara transducing phage DNA, and verified by double digestion with BamI and EcoRI.

MeSH Terms

Bacillus/enzymology Chromosome Mapping Coliphages/analysis,metabolism DNA Restriction Enzymes/metabolism DNA, Viral/analysis,metabolism Endonucleases/metabolism Genes Haemophilus influenzae/enzymology

Chemicals

DNA, Viral Endonucleases DNA Restriction Enzymes

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Haggerty D M

Scheif R F

References (8)

8 references, click to expand

The regulatory region of the L-arabinose operon: its isolation on a 1000 base-pair fragment from DNA heteroduplexes.
J Mol Biol. 1975 Jul 5;95(3):409-16 PMID: 168390
Dual control of arabinose genes on transducing phage lambda-dara.
J Mol Biol. 1971 Jul 14;59(1):127-50 PMID: 4934313
A restriction enzyme from Hemophilus influenzae. I. Purification and general properties.
J Mol Biol. 1970 Jul 28;51(2):379-91 PMID: 5312500
Studies on the cleavage of bacteriophage lambda DNA with EcoRI Restriction endonuclease.
J Mol Biol. 1975 Jan 25;91(3):315-28 PMID: 1102702
Isolation of a sequence-specific endonuclease (BamI) from Bacillus amyloliquefaciens H.
J Mol Biol. 1975 Sep 5;97(1):123-5 PMID: 1177312
The isolation and characterization of plaque-forming arabinose transducing bacteriophage lambda.
J Mol Biol. 1975 Jul 5;95(3):395-407 PMID: 1152060
On the probability of ring closure of lambda DNA.
J Mol Biol. 1966 Aug;19(2):469-82 PMID: 5969076
Thermodynamic and kinetic studies on the interconversion between the linear and circular forms of phage lambda DNA.
J Mol Biol. 1966 Jan;15(1):111-23 PMID: 5912035

Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.18.2.659-663.1976

Published

1976-05-00

Pages

659-63

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC515593

Subset

External Links

PubMed source DOI: 10.1128/JVI.18.2.659-663.1976 PMC Full Text View journal details

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Location in bacteriophage lamdba DNA of cleavage sites of the site-specific endonuclease from Bacillus amyloliquefaciens H.

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