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PMID: 6330042 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Host-dependent transposon Tn5-mediated streptomycin resistance.

Journal of bacteriology ·Vol. 159 ·No. 1 ·1984-07-00 ·Pages 395-9

De Vos GF, Finan TM, Signer ER, Walker GC

Abstract

Transposon Tn5 encodes streptomycin resistance in addition to kanamycin-neomycin resistance. This resistance was not detectable in Escherichia coli but was efficiently expressed in Rhizobium meliloti and certain other strains. By analysis of cloned Tn5 restriction endonuclease fragments, the streptomycin resistance (str) gene was located in the right-hand side of the central region as the transposon is conventionally drawn. Transcription of str appeared to originate at pL, the promoter for the neo gene (neomycin phosphotransferase type II). Expression of streptomycin resistance in E. coli was obtained after cloning of the neo-str region downstream of a strong E. coli promoter. A construct in which PL was deleted also showed differential expression of streptomycin resistance.

MeSH Terms

Cloning, Molecular DNA Restriction Enzymes DNA Transposable Elements Drug Resistance, Microbial Escherichia coli/drug effects,genetics Klebsiella pneumoniae/drug effects,genetics Plasmids Rhizobium/drug effects,genetics Species Specificity Streptomycin/toxicity Transcription, Genetic

Chemicals

DNA Transposable Elements DNA Restriction Enzymes Streptomycin

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

De Vos G F

Finan T M

Signer E R

Walker G C

References (28)

28 references, click to expand

R factor transfer in Rhizobium leguminosarum.
J Gen Microbiol. 1974 Sep;84(1):188-98 PMID: 4612098
Identification of the rhizobium strains in pea root nodules using genetic markers.
J Gen Microbiol. 1975 Apr;87(2):343-50 PMID: 1141859
A freeze-squeeze method for recovering long DNA from agarose gels.
Anal Biochem. 1975 May 26;66(1):213-20 PMID: 1096670
Transposition of R factor genes to bacteriophage lambda.
Proc Natl Acad Sci U S A. 1975 Sep;72(9):3628-32 PMID: 1059152
Construction and characterization of new cloning vehicles. I. Ampicillin-resistant derivatives of the plasmid pMB9.
Gene. 1977;2(2):75-93 PMID: 344136
Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in trans.
Proc Natl Acad Sci U S A. 1979 Apr;76(4):1648-52 PMID: 377280
Complete nucleotide sequence of the Escherichia coli plasmid pBR322.
Cold Spring Harb Symp Quant Biol. 1979;43 Pt 1:77-90 PMID: 383387
A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PMID: 388356
A restriction enzyme cleavage map of Tn5 and location of a region encoding neomycin resistance.
Mol Gen Genet. 1979;177(1):65-72 PMID: 231729
The inverted repeats of Tn5 are functionally different.
Cell. 1980 Mar;19(3):795-805 PMID: 6244898
Genetic organization of the broad-host-range IncP-1 plasmid R751.
J Bacteriol. 1980 Sep;143(3):1362-73 PMID: 6251029
Localization of the Tn5 transposase promoter using the cycling reaction of RNA polymerase.
Nucleic Acids Res. 1981 Apr 24;9(8):1873-83 PMID: 6264398
Succinate transport in Rhizobium leguminosarum.
J Bacteriol. 1981 Oct;148(1):193-202 PMID: 7287623
Structural analysis of Tn5.
Cold Spring Harb Symp Quant Biol. 1981;45 Pt 1:107-13 PMID: 6271452
Insertion, excision, and inversion of Tn5.
Cold Spring Harb Symp Quant Biol. 1981;45 Pt 1:115-23 PMID: 6271454
Analysis of the structure and function of the kanamycin-resistance transposon Tn903.
Cold Spring Harb Symp Quant Biol. 1981;45 Pt 1:125-33 PMID: 6271455
Genetic organization of Tn5.
Cold Spring Harb Symp Quant Biol. 1981;45 Pt 1:99-105 PMID: 6271497
Identification of the uvrA gene product.
J Mol Biol. 1981 May 5;148(1):45-62 PMID: 6273577
Physical and genetic characterization of symbiotic and auxotrophic mutants of Rhizobium meliloti induced by transposon Tn5 mutagenesis.
J Bacteriol. 1982 Jan;149(1):114-22 PMID: 6274841
Analysis of the Inc P-1 group plasmids R906 and R751 and their relationship to RP1.
Plasmid. 1982 Nov;8(3):244-52 PMID: 6294708
Nucleotide sequence and exact localization of the neomycin phosphotransferase gene from transposon Tn5.
Gene. 1982 Oct;19(3):327-36 PMID: 6295884
Localization of symbiotic mutations in Rhizobium meliloti.
J Bacteriol. 1983 Feb;153(2):635-43 PMID: 6296048
The tac promoter: a functional hybrid derived from the trp and lac promoters.
Proc Natl Acad Sci U S A. 1983 Jan;80(1):21-5 PMID: 6337371
Tn5 transposition and its regulation.
Cell. 1982 Dec;31(2 Pt 1):307-8 PMID: 6297758
Proteins required for ultraviolet light and chemical mutagenesis. Identification of the products of the umuC locus of Escherichia coli.
J Mol Biol. 1983 Feb 25;164(2):175-92 PMID: 6302271
Tn5 carries a streptomycin resistance determinant downstream from the kanamycin resistance gene.
Mol Gen Genet. 1983;191(2):288-94 PMID: 6312272
General transduction in Rhizobium meliloti.
J Bacteriol. 1984 Jul;159(1):120-4 PMID: 6330024
Molecular cloning of overlapping segments of the nopaline Ti-plasmid pTiC58 as a means to restriction endonuclease mapping.
Plasmid. 1980 Mar;3(2):193-211 PMID: 6100893

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.159.1.395-399.1984

Published

1984-07-00

Pages

395-9

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC215646

Subset

Grants

NIGMS NIH HHS · GM31030 · United States

External Links

PubMed source DOI: 10.1128/jb.159.1.395-399.1984 PMC Full Text View journal details

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