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PMID: 6330024 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

General transduction in Rhizobium meliloti.

Journal of bacteriology ·Vol. 159 ·No. 1 ·1984-07-00 ·Pages 120-4

Finan TM, Hartweig E, LeMieux K, Bergman K, Walker GC, Signer ER

Abstract

General transduction by phage phi M12 in Rhizobium meliloti SU47 and its derivatives is described. Cotransduction and selection for Tn5 insertions which are closely linked to specific loci were demonstrated. A derivative of SU47 carrying the recA::Tn5 allele of R. meliloti 102F34 could be transduced for plasmid R68.45 but not for chromosomally located alleles. Phage phi M12 is morphologically similar to Escherichia coli phage T4, and restriction endonuclease analysis indicated that the phage DNA was ca. 160 kilobases in size.

MeSH Terms
Bacteriophages/genetics DNA Transposable Elements Escherichia coli/genetics Genotype Plasmids Rhizobium/genetics Transduction, Genetic
Chemicals
DNA Transposable Elements
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Finan T M
Hartweig E
LeMieux K
Bergman K
Walker G C
Signer E R
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25 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.159.1.120-124.1984
Published
1984-07-00
Pages
120-4
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC215601
Subset
IM
Grants
NIGMS NIH HHS · GM31030 · United States
External Links
PubMed source DOI: 10.1128/jb.159.1.120-124.1984 PMC Full Text View journal details
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