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PMID: 6312272 Published · ppublish English Comparative Study Journal Article

Tn5 carries a streptomycin resistance determinant downstream from the kanamycin resistance gene.

Molecular & general genetics : MGG ·Vol. 191 ·No. 2 ·1983-00-00 ·Pages 288-94

Putnoky P, Kiss GB, Ott I, Kondorosi A

Abstract

In Rhizobium meliloti, Tn5 conferred resistance not only to kanamycin but to streptomycin, as well, in Escherichia coli, however only to kanamycin. Using in vitro recombinant DNA techniques, it was shown that the streptomycin resistance determinant was located downstream from the kanamycin resistance gene in the unique central region of Tn5. Expression of various cloned fragments of Tn5 suggested that both kanamycin and streptomycin resistance genes were transcribed from the same promoter. E. coli mutants allowing the expression of streptomycin resistance from Tn5 were isolated. The differential expression of the streptomycin resistance gene provides a simple selection/counterselection criterion, using only streptomycin in transfer experiments of Tn5 between E. coli and R. meliloti.

MeSH Terms
Chromosome Mapping DNA Transposable Elements DNA, Recombinant Drug Resistance, Microbial Escherichia coli/genetics Genes, Bacterial Kanamycin/pharmacology Mutation Operon Plasmids Rhizobium/genetics Streptomycin/pharmacology
Chemicals
DNA Transposable Elements DNA, Recombinant Kanamycin Streptomycin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Putnoky P
Kiss G B
Ott I
Kondorosi A
References (33)
33 references, click to expand
  1. Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti.
    Proc Natl Acad Sci U S A. 1980 Dec;77(12):7347-51 PMID: 7012838
  2. Model for regulation of the histidine operon of Salmonella.
    Proc Natl Acad Sci U S A. 1980 Jan;77(1):508-12 PMID: 6987654
  3. Insertion, excision, and inversion of Tn5.
    Cold Spring Harb Symp Quant Biol. 1981;45 Pt 1:115-23 PMID: 6271454
  4. Analysis of endonuclease R-EcoRI fragments of DNA from lambdoid bacteriophages and other viruses by agarose-gel electrophoresis.
    J Virol. 1974 Nov;14(5):1235-44 PMID: 4372397
  5. Release of polarity in Escherichia coli by gene N of phage lambda: termination and antitermination of transcription.
    Proc Natl Acad Sci U S A. 1974 Jun;71(6):2534-8 PMID: 4601822
  6. Uniform nomenclature for bacterial plasmids: a proposal.
    Bacteriol Rev. 1976 Mar;40(1):168-89 PMID: 1267736
  7. Expression of a transposable antibiotic resistance element in Saccharomyces.
    Nature. 1980 Oct 30;287(5785):869-71 PMID: 6253817
  8. Genetic organization of Tn5.
    Cold Spring Harb Symp Quant Biol. 1981;45 Pt 1:99-105 PMID: 6271497
  9. A new dominant hybrid selective marker for higher eukaryotic cells.
    J Mol Biol. 1981 Jul 25;150(1):1-14 PMID: 6271971
  10. Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA.
    Proc Natl Acad Sci U S A. 1972 Aug;69(8):2110-4 PMID: 4559594
  11. Plasmid vehicles for direct cloning of Escherichia coli promoters.
    J Bacteriol. 1979 Nov;140(2):400-7 PMID: 387734
  12. Transposition of R factor genes to bacteriophage lambda.
    Proc Natl Acad Sci U S A. 1975 Sep;72(9):3628-32 PMID: 1059152
  13. Transposable elements.
    Cell. 1980 Jul;20(3):579-95 PMID: 6251969
  14. Properties of a supercoiled deoxyribonucleic acid-protein relaxation complex and strand specificity of the relaxation event.
    Biochemistry. 1970 Oct 27;9(22):4428-40 PMID: 4919547
  15. The functional differences in the inverted repeats of Tn5 are caused by a single base pair nonhomology.
    Cell. 1981 Jan;23(1):191-9 PMID: 6260374
  16. Rifampicin supersensitivity of rho strains of E. coli, and suppression by sur mutation.
    Mol Gen Genet. 1979 Jan 16;169(1):27-34 PMID: 374996
  17. A dye-buoyant-density method for the detection and isolation of closed circular duplex DNA: the closed circular DNA in HeLa cells.
    Proc Natl Acad Sci U S A. 1967 May;57(5):1514-21 PMID: 5231757
  18. An intercistronic region and ribosome-binding site in bacterial messenger RNA.
    Proc Natl Acad Sci U S A. 1975 Jun;72(6):2399-403 PMID: 1094468
  19. New map of bacteriophage lambda DNA.
    J Virol. 1980 Jan;33(1):390-400 PMID: 6245240
  20. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
    Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PMID: 388356
  21. Nucleotide sequence of the R.meliloti nitrogenase reductase (nifH) gene.
    Nucleic Acids Res. 1981 Nov 11;9(21):5711-23 PMID: 6273806
  22. A restriction enzyme cleavage map of Tn5 and location of a region encoding neomycin resistance.
    Mol Gen Genet. 1979;177(1):65-72 PMID: 231729
  23. Recalibrated linkage map of Escherichia coli K-12.
    Bacteriol Rev. 1976 Mar;40(1):116-67 PMID: 773363
  24. The inverted repeats of Tn5 are functionally different.
    Cell. 1980 Mar;19(3):795-805 PMID: 6244898
  25. Evidence that ribosomal protein S10 participates in control of transcription termination.
    Proc Natl Acad Sci U S A. 1981 Feb;78(2):1115-8 PMID: 6453343
  26. Isolation of a temperature-sensitive derivative of RP1.
    Plasmid. 1980 May;3(3):343-7 PMID: 6100900
  27. Structural analysis of Tn5.
    Cold Spring Harb Symp Quant Biol. 1981;45 Pt 1:107-13 PMID: 6271452
  28. A proposal for a uniform nomenclature in bacterial genetics.
    Genetics. 1966 Jul;54(1):61-76 PMID: 5961488
  29. Physical and genetic characterization of symbiotic and auxotrophic mutants of Rhizobium meliloti induced by transposon Tn5 mutagenesis.
    J Bacteriol. 1982 Jan;149(1):114-22 PMID: 6274841
  30. Genetic studies on rhizobiophage 16-3. I. Genes and functions on the chromosome.
    Mol Gen Genet. 1973 Sep 27;125(4):341-50 PMID: 4777790
  31. Bacterial mutants in which the gene N function of bacteriophage lambda is blocked have an altered RNA polymerase.
    Proc Natl Acad Sci U S A. 1971 Dec;68(12):2977-81 PMID: 4943550
  32. Nucleotide sequence and exact localization of the neomycin phosphotransferase gene from transposon Tn5.
    Gene. 1982 Oct;19(3):327-36 PMID: 6295884
  33. Localization of symbiotic mutations in Rhizobium meliloti.
    J Bacteriol. 1983 Feb;153(2):635-43 PMID: 6296048
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00334828
Published
1983-00-00
Pages
288-94
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00334828
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