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PMID: 6327612 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Transposon Tn5 specifies streptomycin resistance in Rhizobium spp.

Journal of bacteriology ·Vol. 158 ·No. 2 ·1984-05-00 ·Pages 580-9

Selvaraj G, Iyer VN

Abstract

Transposon Tn5 conferred streptomycin resistance on different strains of Rhizobium meliloti, Rhizobium leguminosarum, and Rhizobium trifolii but not on Escherichia coli. A gene (str) specifying this phenotype has been identified and localized on the physical and genetic map of Tn5. It is transcribed from the promoter of neo, the gene that encodes neomycin phosphotransferase. The str gene is downstream from neo in a single transcriptional unit, as revealed by molecular cloning of different segments of Tn5 and by cloning of the neo-str region of Tn5 downstream from a lac promoter. Fusion of the SalI-generated rightward segment of Tn5 (devoid of neo) to a part of a tetracycline resistance gene, tet, in a plasmid or downstream from a lac promoter in a plasmid resulted in significant levels of streptomycin resistance in an R. meliloti host, suggesting that the str gene product can function independent of neomycin phosphotransferase. A natural isolate of R. meliloti that does not express Tn5-associated streptomycin resistance has been identified. We have used the str of Tn5 as a genetic marker in Rhizobium spp.

MeSH Terms
DNA Transposable Elements DNA, Recombinant Drug Resistance, Microbial Genes, Bacterial Genetic Markers Kanamycin/pharmacology Kanamycin Kinase Operon Phosphotransferases/genetics Rhizobium/drug effects,genetics Streptomycin/pharmacology Transcription, Genetic
Chemicals
DNA Transposable Elements DNA, Recombinant Genetic Markers Kanamycin Phosphotransferases Kanamycin Kinase Streptomycin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Selvaraj G
Iyer V N
References (32)
32 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.158.2.580-589.1984
Published
1984-05-00
Pages
580-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC215468
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.158.2.580-589.1984 PMC Full Text View journal details
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