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PMID: 393953 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

The DNA-protein relaxation complex of the plasmid RK2: location of the site-specific nick in the region of the proposed origin of transfer.

Molecular & general genetics : MGG ·Vol. 176 ·No. 2 ·1979-10-03 ·Pages 183-9

Guiney DG, Helinski DR

Abstract

The broad jost range plasmid, RK2, has been isolated as a DNA-protein relaxation complex. Nicking of the plasmid DNA in the relaxation complex occurs at a single specific site (rlx) located approximately 20 kb away from the origin of DNA replication. A cis-acting function required for plasmid transfer, the presumptive origin of transfer, maps in the same region as rlx. The region of RK2 encompassing rlx has been cloned onto pBR322 and shown to promote mobilization of the hybrid plasmid by an RK2 derivative. These results indicate that the RK2 relaxation complex nicks at or near the origin of transfer of the RK2 plasmid.

MeSH Terms
Bacterial Proteins/metabolism Chromosome Mapping Cloning, Molecular Conjugation, Genetic DNA Replication DNA, Superhelical/physiology Escherichia coli/genetics Macromolecular Substances Protein Binding R Factors Transformation, Genetic
Chemicals
Bacterial Proteins DNA, Superhelical Macromolecular Substances
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Guiney D G
Helinski D R
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25 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00273212
Published
1979-10-03
Pages
183-9
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00273212
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