Home LiteratureArticle Details
PMID: 3513123 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Promoters of Agrobacterium tumefaciens Ti-plasmid virulence genes.

Nucleic acids research ·Vol. 14 ·No. 3 ·1986-02-11 ·Pages 1355-64

Das A, Stachel S, Ebert P, Allenza P, Montoya A, Nester E

Abstract

The DNA sequences of the promoter and 5' upstream regions of six Agrobacterium tumefaciens Ti-plasmid encoded virulence (vir) genes were determined. The transcription initiation sites were mapped by the S1 nuclease protection assay. In the -10 region, the vir promoters share a consensus sequence that is homologous to a DNA sequence found in the same region of E. coli promoters. In contrast, the -35 region sequences are variable. Several vir genes contain two common hexanucleotide sequences, 5'CGAGTA3' and 5'GCAATT3'. Translation initiation codons for all vir genes, except virG, are preceded by sequences homologous to the ribosome binding site sequences found in E. coli.

MeSH Terms
Base Sequence Escherichia coli/genetics Gene Expression Regulation Genes, Bacterial Plant Tumors/etiology Plasmids Promoter Regions, Genetic Rhizobium/genetics,pathogenicity Sequence Homology, Nucleic Acid
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Das A
Stachel S
Ebert P
Allenza P
Montoya A
Nester E
References (21)
21 references, click to expand
  1. The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites.
    Proc Natl Acad Sci U S A. 1974 Apr;71(4):1342-6 PMID: 4598299
  2. The molecular genetics of crown gall tumorigenesis.
    Adv Genet. 1984;22:209-83 PMID: 15633289
  3. Completed DNA sequences and organization of repressor-binding sites in the operators of phage lambda.
    J Mol Biol. 1977 May 15;112(2):265-77 PMID: 875019
  4. Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.
    Cell. 1977 Nov;12(3):721-32 PMID: 922889
  5. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  6. DNA sequence of the araBAD promoter in Escherichia coli B/r.
    Proc Natl Acad Sci U S A. 1978 Oct;75(10):4724-8 PMID: 368797
  7. Regulatory sequences involved in the promotion and termination of RNA transcription.
    Annu Rev Genet. 1979;13:319-53 PMID: 94251
  8. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  9. Transcription maps of polyoma virus-specific RNA: analysis by two-dimensional nuclease S1 gel mapping.
    Methods Enzymol. 1980;65(1):718-49 PMID: 6154876
  10. Agrobacterium tumefaciens mutants affected in crown gall tumorigenesis and octopine catabolism.
    J Bacteriol. 1980 Nov;144(2):732-43 PMID: 6253441
  11. Nucleotide sequence and expression of Escherichia coli trpR, the structural gene for the trp aporepressor.
    Proc Natl Acad Sci U S A. 1980 Dec;77(12):7117-21 PMID: 7012834
  12. Evidence for two functional gal promoters in intact Escherichia coli cells.
    J Biol Chem. 1981 Nov 25;256(22):11905-10 PMID: 6271763
  13. Complementation analysis of Agrobacterium tumefaciens Ti plasmid mutations affecting oncogenicity.
    J Bacteriol. 1982 Apr;150(1):327-31 PMID: 7061395
  14. Trp aporepressor production is controlled by autogenous regulation and inefficient translation.
    Proc Natl Acad Sci U S A. 1982 May;79(10):3120-4 PMID: 7048301
  15. A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments.
    Gene. 1982 Oct;19(3):269-76 PMID: 6295880
  16. Mutational analysis of the virulence region of an Agrobacterium tumefaciens Ti plasmid.
    J Bacteriol. 1983 Feb;153(2):878-83 PMID: 6296058
  17. Cyclic AMP receptor protein: role in transcription activation.
    Science. 1984 May 25;224(4651):831-8 PMID: 6372090
  18. Plant-inducible virulence promoter of the Agrobacterium tumefaciens Ti plasmid.
    Nature. 1984 Dec 6-12;312(5994):564-6 PMID: 6504164
  19. Identification of pTiC58 plasmid-encoded proteins for virulence in Agrobacterium tumefaciens.
    Proc Natl Acad Sci U S A. 1985 May;82(9):2669-73 PMID: 2986128
  20. A Tn3 lacZ transposon for the random generation of beta-galactosidase gene fusions: application to the analysis of gene expression in Agrobacterium.
    EMBO J. 1985 Apr;4(4):891-8 PMID: 2990912
  21. Agrobacterium tumefaciens DNA and PS8 bacteriophage DNA not detected in crown gall tumors.
    Proc Natl Acad Sci U S A. 1974 Sep;71(9):3672-6 PMID: 4530328
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/14.3.1355
Published
1986-02-11
Pages
1355-64
Language
English
Region
England
NLM ID
0411011
PMCID
PMC339509
Subset
IM
Grants
NIGMS NIH HHS · GM-32618-14 · United States
NIGMS NIH HHS · GM09050-02 · United States
Databases
GENBANK
X03511, X03512, X03513, X03514, X03515, X03516
External Links
PubMed source DOI: 10.1093/nar/14.3.1355 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2