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PMID: 2103448 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Agrobacterium-mediated plant transformation by novel mini-T vectors in conjunction with a high-copy vir region helper plasmid.

Plant molecular biology ·Vol. 15 ·No. 2 ·1990-08-00 ·Pages 245-56

Zyprian E, Kado CI

Abstract

A new binary vector system for Agrobacterium-mediated plant transformation was developed. A set of four mini-T vectors comprised of T-DNA border sequences from nopaline-type Ti-plasmid pTiC58 flanking a chimaeric hygromycin-resistance gene for selection of transformants and up to eight unique restriction sites for cloning foreign DNA was constructed on a broad-host replicon containing the oriV of plasmid pSa. In two of the constructs these multiple cloning sites are flanked by a strong promoter to activate transcription of inserted DNA in planta. High-efficiency transformation was prompted by a high-copy, stable virulence helper plasmid pUCD2614, which contains a cloned virulence region of pTiC58 and tandem copies of the par locus of plasmid pTAR. Southern blot hybridization and genetic analyses of the progeny of transformed plants showed that the hygromycin resistance gene was stably inherited.

Related Genes
Pnos hph nptII oriV par vir
MeSH Terms
Cloning, Molecular/methods DNA, Bacterial/genetics Drug Resistance Genetic Vectors Hygromycin B/pharmacology Plants, Toxic Plasmids Rhizobium/genetics Tobacco/drug effects,genetics Transformation, Genetic
Chemicals
DNA, Bacterial Hygromycin B
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Zyprian E
Department of Plant Pathology, University of California, Davis 95616.
Kado C I
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32 references, click to expand
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Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1007/BF00036911
Published
1990-08-00
Pages
245-56
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00036911 View journal details
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