Bleomycin resistance: a new dominant selectable marker for plant cell transformation.

Plant molecular biology ·Vol. 7 ·No. 3 ·1986-05-00 ·Pages 171-6

Hille J, Verheggen F, Roelvink P, Franssen H, van Kammen A, Zabel P

Abstract

Plant cells are sensitive to the antibiotic bleomycin, a DNA damaging glycopeptide. A bleomycin resistance determinant, located on transposon Tn5 and functional in bacteria, has been cloned in a plant expression vector and introduced into Nicotiana plumbaginifolia using Agrobacterium tumefaciens. The expression of this determinant in plant cells confers resistance to bleomycin and allows selection of transformed plant cells.

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Hille J

Department of Molecular Biology, Agricultural University, De Dreijen 11, 6703 BC, Wageningen, The Netherlands.

Verheggen F

Roelvink P

Franssen H

van Kammen A

Zabel P

References (20)

20 references, click to expand

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Article Info

Journal

Plant molecular biology

Abbr.

Plant Mol Biol

ISSN

0167-4412

DOI

10.1007/BF00021328

Published

1986-05-00

Pages

171-6

Language

English

Region

Netherlands

NLM ID

9106343

External Links

PubMed source DOI: 10.1007/BF00021328 View journal details

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Bleomycin resistance: a new dominant selectable marker for plant cell transformation.

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