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PMID: 16453629 Published · ppublish English Journal Article

Expression of tobacco mosaic virus coat protein by a cauliflower mosaic virus promoter in plants transformed by Agrobacterium.

The EMBO journal ·Vol. 4 ·No. 8 ·1985-08-00 ·Pages 1921-6

Bevan MW, Mason SE, Goelet P

Abstract

The 35S transcript promoter of cauliflower mosaic virus directs the synthesis of an abundant RNA in infected leaf tissue that is both a template for reverse transcription during virus replication and also a polycistronic mRNA. The 35S promoter was isolated and linked to a cDNA of the 3' end of tobacco mosaic virus that encodes the coat protein of this single-stranded RNA virus. After transformation of this gene construction into tobacco plants using a disarmed Agrobacterium binary vector, viral coat protein synthesis was detected in transformed leaf tissue. A chimaeric 35S coat protein RNA was detected in transformed callus.

Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Bevan M W
Plant Breeding Institute, Maris Lane, Trumpington, Cambridge CB2 2LQ, UK.
Mason S E
Goelet P
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1985-08-00
Pages
1921-6
Language
English
Region
England
NLM ID
8208664
PMCID
PMC554441
External Links
PubMed source PMC Full Text View journal details
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