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PMID: 6087294 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The use of heparin as a simple cost-effective means of controlling background in nucleic acid hybridization procedures.

Nucleic acids research ·Vol. 12 ·No. 14 ·1984-07-25 ·Pages 5627-38

Singh L, Jones KW

Abstract

The use of heparin as a simple, effective and cheap substitute for conventional methods of controlling background in hybridization procedures is described and illustrated with reference to the use of DNA probes in filter and in situ hybridization. Possible specific mechanisms for this heparin effect are discussed.

MeSH Terms

Animals DNA Restriction Enzymes Female Heparin Male Mice Nucleic Acid Hybridization

Chemicals

Heparin DNA Restriction Enzymes

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Singh L

Jones K W

References (17)

17 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/12.14.5627

Published

1984-07-25

Pages

5627-38

Language

English

Region

England

NLM ID

0411011

PMCID

PMC320019

Subset

External Links

PubMed source DOI: 10.1093/nar/12.14.5627 PMC Full Text View journal details

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The use of heparin as a simple cost-effective means of controlling background in nucleic acid hybridization procedures.

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