A rapid micro scale method for the detection of lysopine and nopaline dehydrogenase activities.

Biochimica et biophysica acta ·Vol. 527 ·No. 2 ·1978-12-08 ·Pages 497-500

Otten LA, Schilperoort RA

Abstract

A rapid and sensitive method has been developed to determine lysopine dehydrogenase (EC 1.5.1-) and nopaline dehydrogenase activities in crown gall tumour tissues. By this method, enzyme activities as low as 0.2 micrometerol octopine or nopaline per h per g fresh weight tumour tissue can still be detected. In non-infected young pea seedlings, no lysopine dehydrogenase activity was detected.

MeSH Terms

Arginine/analogs & derivatives D-Amino-Acid Oxidase/analysis Glutarates Lysine/analogs & derivatives Microchemistry/methods Oxidoreductases Acting on CH-NH Group Donors/analysis Plant Tumors/enzymology Rhizobium

Chemicals

Glutarates nopaline lysopine Arginine D-Amino-Acid Oxidase Oxidoreductases Acting on CH-NH Group Donors nopaline dehydrogenase Lysine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Otten L A

Schilperoort R A

Article Info

Journal

Biochimica et biophysica acta

Abbr.

Biochim Biophys Acta

ISSN

0006-3002

DOI

10.1016/0005-2744(78)90363-7

Published

1978-12-08

Pages

497-500

Language

English

Region

Netherlands

NLM ID

0217513

Subset

External Links

PubMed source DOI: 10.1016/0005-2744(78)90363-7

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A rapid micro scale method for the detection of lysopine and nopaline dehydrogenase activities.

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