Home LiteratureArticle Details
PMID: 16593241 Published · ppublish English Journal Article

Short direct repeats flank the T-DNA on a nopaline Ti plasmid.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 20 ·1982-10-00 ·Pages 6322-6

Yadav NS, Vanderleyden J, Bennett DR, Barnes WM, Chilton MD

Abstract

Crown gall disease results from the insertion of a segment of the Agrobacterium Ti plasmid, called T-DNA, into host plant nuclear DNA. We have subjected to sequence analysis the border regions of pTi T37 (ends of T-DNA) and one left T-DNA/plant DNA border fragment isolated from BT37 tobacco teratoma by molecular cloning. These sequence studies, taken together with published sequence of a right T-DNA/plant DNA border fragment, allowed us to identify the positions of left and right borders at the DNA sequence level. Comparison of left and right border regions of the Ti plasmid revealed a "core" direct repeat of 13 of 14 bases (12 contiguous) precisely at the borders of T-DNA. An extended repeat of 21 of 25 bases overlaps this core repeat. T-DNA on the Ti plasmid exhibits no longer direct or inverted repeats in the border regions, based on Southern hybridization studies. The physical structure of T-DNA differs from that of known prokaryotic and eukaryotic transposable elements but bears a structural resemblance to the prophage of bacteriophage lambda.

Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Yadav N S
Department of Biology, Washington University, St. Louis, Missouri 63130.
Vanderleyden J
Bennett D R
Barnes W M
Chilton M D
References (49)
49 references, click to expand
  1. Proteolytic cleavage fo native DNA polymerase into two different catalytic fragments. Influence of assay condtions on the change of exonuclease activity and polymerase activity accompanying cleavage.
    Eur J Biochem. 1971 Oct 14;22(3):371-81 PMID: 4942147
  2. Octopine and nopaline metabolism in Agrobacterium tumefaciens and crown gall tumor cells: role of plasmid genes.
    J Bacteriol. 1977 Jan;129(1):101-7 PMID: 830636
  3. Large plasmid in Agrobacterium tumefaciens essential for crown gall-inducing ability.
    Nature. 1974 Nov 8;252(5479):169-70 PMID: 4419109
  4. Deoxyribonucleic acid polymerase: two distinct enzymes in one polypeptide. I. A proteolytic fragment containing the polymerase and 3' leads to 5' exonuclease functions.
    J Biol Chem. 1972 Jan 10;247(1):224-31 PMID: 4552924
  5. Isolation of the bacteriophage lambda A-gene protein.
    Proc Natl Acad Sci U S A. 1975 Feb;72(2):581-5 PMID: 1054839
  6. Octopine and nopaline synthesis and breakdown genetically controlled by a plasmid of Agrobacterium tumefaciens.
    Mol Gen Genet. 1976 May 7;145(2):177-81 PMID: 934053
  7. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  8. Plasmid required for virulence of Agrobacterium tumefaciens.
    J Bacteriol. 1975 Jul;123(1):255-64 PMID: 1141196
  9. Recombination between higher plant DNA and the Ti plasmid of Agrobacterium tumefaciens.
    Proc Natl Acad Sci U S A. 1980 Nov;77(11):6448-52 PMID: 16592915
  10. T-DNA from Agrobacterium Ti plasmid is in the nuclear DNA fraction of crown gall tumor cells.
    Proc Natl Acad Sci U S A. 1980 Jul;77(7):4060-4 PMID: 16592850
  11. Agropine in "null-type" crown gall tumors: Evidence for generality of the opine concept.
    Proc Natl Acad Sci U S A. 1980 May;77(5):2693-7 PMID: 16592823
  12. Proteins encoded by Agrobacterium tumefaciens Ti plasmid DNA (T-DNA) in crown gall tumors.
    Proc Natl Acad Sci U S A. 1980 May;77(5):2666-70 PMID: 16592819
  13. A PLANT-TUMOR OF BACTERIAL ORIGIN.
    Science. 1907 Apr 26;25(643):671-3 PMID: 17746161
  14. [ON THE PRESENCE OF OCTINE IN CROWN-GALL].
    C R Hebd Seances Acad Sci. 1964 Dec 21;259:4795-6 PMID: 14254937
  15. Molecular cloning of overlapping segments of the nopaline Ti-plasmid pTiC58 as a means to restriction endonuclease mapping.
    Plasmid. 1980 Mar;3(2):193-211 PMID: 6100893
  16. Genetic analysis of crown gall: fine structure map of the T-DNA by site-directed mutagenesis.
    Cell. 1981 Nov;27(1 Pt 2):143-53 PMID: 6276020
  17. Characterization of Tn904 insertions in octopine Ti plasmid mutants of Agrobacterium tumefaciens.
    J Bacteriol. 1980 Oct;144(1):82-91 PMID: 6252198
  18. Origin of retroviruses from cellular moveable genetic elements.
    Cell. 1980 Oct;21(3):599-600 PMID: 6254661
  19. Transposable elements.
    Cell. 1980 Jul;20(3):579-95 PMID: 6251969
  20. Agrobacterium tumefaciens mutants affected in crown gall tumorigenesis and octopine catabolism.
    J Bacteriol. 1980 Nov;144(2):732-43 PMID: 6253441
  21. Terminal repeats of the Drosophila transposable element copia: nucleotide sequence and genomic organization.
    Cell. 1980 Sep;21(2):581-8 PMID: 6250726
  22. Insertion of the Drosophila transposable element copia generates a 5 base pair duplication.
    Cell. 1980 Sep;21(2):575-9 PMID: 6250725
  23. The yeast transposon Ty1 generates duplications of target DNA on insertion.
    Nature. 1980 Jul 24;286(5771):414-8 PMID: 6250065
  24. Insertion of the eukaryotic transposable element Ty1 creates a 5-base pair duplication.
    Nature. 1980 Jul 24;286(5771):352-6 PMID: 6250062
  25. A system for shotgun DNA sequencing.
    Nucleic Acids Res. 1981 Jan 24;9(2):309-21 PMID: 6259625
  26. Secondary lambda attachment site in the threonine operon attenuator of Escherichia coli.
    J Bacteriol. 1981 Jun;146(3):1046-54 PMID: 6263855
  27. The boundaries and copy numbers of Ti plasmid T-DNA vary in crown gall tumors.
    Mol Gen Genet. 1980;177(4):637-43 PMID: 6247611
  28. Extensive transcription of foreign DNA in a crown gall teratoma.
    Biochem Biophys Res Commun. 1980 Feb 27;92(4):1273-7 PMID: 6245650
  29. Tumor DNA structure in plant cells transformed by A. tumefaciens.
    Science. 1980 Sep 19;209(4463):1385-91 PMID: 6251546
  30. Structure of chi hotspots of generalized recombination.
    Cell. 1981 May;24(2):429-36 PMID: 6453653
  31. Directionality and nonreciprocality of Chi-stimulated recombination in phage lambda.
    Genetics. 1980 Feb;94(2):235-48 PMID: 6446478
  32. Integration and excision of bacteriophage lambda: the mechanism of conservation site specific recombination.
    Annu Rev Genet. 1981;15:143-67 PMID: 6461289
  33. Site-specific mutagenesis of Agrobacterium Ti plasmids and transfer of genes to plant cells.
    J Mol Appl Genet. 1981;1(2):149-64 PMID: 7050279
  34. Site-specific insertion of genes into T-DNA of the Agrobacterium tumor-inducing plasmid: an approach to genetic engineering of higher plant cells.
    J Mol Appl Genet. 1981;1(1):39-49 PMID: 6955419
  35. Foreign DNA sequences in crown gall teratomas and their fate during the loss of the tumorous traits.
    Mol Gen Genet. 1980;177(4):707-14 PMID: 6929944
  36. DNA from the A6S/2 crown gall tumor contains scrambled Ti-plasmid sequences near its junctions with plant DNA.
    Cell. 1982 Jul;29(3):1005-14 PMID: 7151163
  37. Internal organization, boundaries and integration of Ti-plasmid DNA in nopaline grown gall tumours.
    J Mol Biol. 1980 Dec 15;144(3):353-76 PMID: 7253020
  38. Integration and organization of Ti plasmid sequences in crown gall tumors.
    Cell. 1980 Mar;19(3):729-39 PMID: 7363328
  39. Cloning human fetal gamma globin and mouse alpha-type globin DNA: preparation and screening of shotgun collections.
    Science. 1978 Dec 22;202(4374):1279-84 PMID: 725603
  40. Molecular cloning of the DNA ligase gene from bacteriophage T4. II. Amplification and preparation of the gene product.
    J Mol Biol. 1979 Aug 15;132(3):493-505 PMID: 392109
  41. A rapid procedure for isolation of large quantities of Escherichia coli DNA polymerase I utilizing a lambdapolA transducing phage.
    J Biol Chem. 1979 May 10;254(9):3206-10 PMID: 372187
  42. Synthetic adaptors for cloning DNA.
    Methods Enzymol. 1979;68:98-109 PMID: 232227
  43. Transcription of Ti plasmid-derived sequences in three octopine-type crown gall tumor lines.
    Proc Natl Acad Sci U S A. 1979 Jun;76(6):2828-32 PMID: 379864
  44. Construction and characterization of new cloning vehicles. III. Derivatives of plasmid pBR322 carrying unique Eco RI sites for selection of Eco RI generated recombinant DNA molecules.
    Gene. 1978 Oct;4(2):121-36 PMID: 363519
  45. Viral integration and excision: structure of the lambda att sites.
    Science. 1977 Sep 16;197(4309):1147-60 PMID: 331474
  46. Plasmid detection and sizing in single colony lysates.
    Science. 1977 Jan 28;195(4276):393-4 PMID: 318764
  47. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  48. Stable incorporation of plasmid DNA into higher plant cells: the molecular basis of crown gall tumorigenesis.
    Cell. 1977 Jun;11(2):263-71 PMID: 890735
  49. Supercoiled circular DNA in crown-gall inducing Agrobacterium strains.
    J Mol Biol. 1974 Jun 15;86(1):109-27 PMID: 4854526
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.20.6322
Published
1982-10-00
Pages
6322-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC347113
External Links
PubMed source DOI: 10.1073/pnas.79.20.6322 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2