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PMID: 15966101 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Overdrive, a T-DNA transmission enhancer on the A. tumefaciens tumour-inducing plasmid.

The EMBO journal ·Vol. 5 ·No. 6 ·1986-06-00 ·Pages 1137-42

Peralta EG, Hellmiss R, Ream W

Abstract

During crown gall tumorigenesis a specific segment of the Agrobacterium tumefaciens tumour-inducing (Ti) plasmid, the T-DNA, integrates into plant nuclear DNA. Similar 23-bp direct repeats at each end of the T region signal T-DNA borders, and T-DNA transmission (transfer and integration) requires the right-hand direct repeat. A chemically synthesized right border repeat in its wild-type orientation promotes T-DNA transmission at a low frequency; Ti plasmid sequences which normally flank the right repeat greatly stimulate the process. To identify flanking sequences required for full right border activity, we tested the activity of a border repeat surrounded by different amounts of normal flanking sequences. Efficient T-DNA transmission required a conserved sequence (5' TAAPuTPy-CTGTPuT-TGTTTGTTTG 3') which lies to the right of the two known right border repeats. In either orientation, a synthetic oligonucleotide containing this conserved sequence greatly stimulated the activity of a right border repeat, and a deletion removing 15 bp from the right end of this sequence destroyed it stimulatory effect. Thus, wild-type T-DNA transmission required both the 23-bp right border repeat and a conserved flanking sequence which we call overdrive.

MeSH Terms
Agrobacterium tumefaciens/genetics Base Sequence DNA, Bacterial/genetics Enhancer Elements, Genetic/genetics Genes, Bacterial/genetics Molecular Sequence Data Plant Tumor-Inducing Plasmids/genetics
Chemicals
DNA, Bacterial T-DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Peralta E G
Institute for Molecular Biology, Department of Biology, Indiana University, Bloomington, IN 47405, USA.
Hellmiss R
Ream W
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1986-06-00
Pages
1137-42
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1166919
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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