Genetic Identification of functions of TL-DNA transcripts in octopine crown galls.
The TL-DNA in octopine crown galls encodes seven transcripts most, if not all, expressed from individual promoters. Site-specific deletions and substitutions in the T-region of the octopine plasmid pTiB6S3 indicate some of the functions of the TL-DNA transcripts. Two of the seven genes are sufficient to allow tumorous growth. T-DNA transfer and oncogenicity are controlled by different and independently acting functions. None of the transcripts of TL-DNA appear to be essential for T-DNA transfer. Four, possibly five, of the TL-DNA transcripts act by suppressing organ development. Shoot and root formation are suppressed by the action of different transcripts.
17 references, click to expand
-
The functional organization of the nopaline A. tumefaciens plasmid pTiC58.Plasmid. 1980 Mar;3(2):212-30 PMID: 6100894
-
Restriction endonuclease mapping of the octopine tumor-inducing plasmid pTiAch5 of Agrobacterium tumefaciens.Plasmid. 1981 Sep;6(2):249-53 PMID: 6272339
-
Physical mapping of DNA base sequence homologies between an octopine and a nopaline Ti plasmid of Agrobacterium tumefaciens.J Mol Biol. 1981 Oct 25;152(2):183-208 PMID: 6276566
-
Further insight on the transferred-DNA of octopine crown gall.Mol Gen Genet. 1981;183(2):283-8 PMID: 6276681
-
Site-specific insertion of genes into T-DNA of the Agrobacterium tumor-inducing plasmid: an approach to genetic engineering of higher plant cells.J Mol Appl Genet. 1981;1(1):39-49 PMID: 6955419
-
Internal organization, boundaries and integration of Ti-plasmid DNA in nopaline grown gall tumours.J Mol Biol. 1980 Dec 15;144(3):353-76 PMID: 7253020
-
Integration and organization of Ti plasmid sequences in crown gall tumors.Cell. 1980 Mar;19(3):729-39 PMID: 7363328
-
Recombination between higher plant DNA and the Ti plasmid of Agrobacterium tumefaciens.Proc Natl Acad Sci U S A. 1980 Nov;77(11):6448-52 PMID: 16592915
-
Plasmid pKC7: a vector containing ten restriction endonuclease sites suitable for cloning DNA segments.Gene. 1979 Sep;7(1):79-82 PMID: 159209
-
Large plasmid in Agrobacterium tumefaciens essential for crown gall-inducing ability.Nature. 1974 Nov 8;252(5479):169-70 PMID: 4419109
-
Expression of plant tumor-specific proteins in minicells of Escherichia coli: a fusion protein of lysopine dehydrogenase with chloramphenicol acetyltransferase.Nucleic Acids Res. 1981 Oct 24;9(20):5187-202 PMID: 6117827
-
Tumor DNA structure in plant cells transformed by A. tumefaciens.Science. 1980 Sep 19;209(4463):1385-91 PMID: 6251546
-
Genetic analysis of crown gall: fine structure map of the T-DNA by site-directed mutagenesis.Cell. 1981 Nov;27(1 Pt 2):143-53 PMID: 6276020
-
Mendelian transmission of genes introduced into plants by the Ti plasmids of Agrobacterium tumefaciens.Mol Gen Genet. 1981;183(2):209-13 PMID: 6948997
-
Site-specific mutagenesis of Agrobacterium Ti plasmids and transfer of genes to plant cells.J Mol Appl Genet. 1981;1(2):149-64 PMID: 7050279
-
The TL-DNA in octopine crown-gall tumours codes for seven well-defined polyadenylated transcripts.EMBO J. 1982;1(1):139-46 PMID: 16453403
-
Grown gall plant tumors of abnormal morphology, induced by Agrobacterium tumefaciens carrying mutated octopine Ti plasmids; analysis of T-DNA functions.Gene. 1981 Jun-Jul;14(1-2):33-50 PMID: 6266929
Contact
No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong
Qilu Normal University · Genelibs Bioinformatics Lab
750 Shunhua Rd, Jinan
2F, Bldg F, University Science Park
Tel: 0531-88819269
WeChat Official Account
Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.
Business Email
E-mail: product@genelibs.com
Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2
