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PMID: 16453403 Published · ppublish English Journal Article

The TL-DNA in octopine crown-gall tumours codes for seven well-defined polyadenylated transcripts.

The EMBO journal ·Vol. 1 ·No. 1 ·1982-00-00 ·Pages 139-46

Willmitzer L, Simons G, Schell J

Abstract

Seven polyadenylated transcripts of significantly different relative abundance were detected in octopine crown-gall tissue after gel electrophoretic separation and subsequent transfer to diazobenzyloxymethyl paper. The transcripts range from 670 to 2700 bases long. The different transcripts were located using 19 different fragments of the TL-region as probes. By hybridizing labelled RNA to separated complementary strands of the T-DNA, and parallel determination of the chemical polarity of the strands, the 5' - 3' orientations of six of the seven transcripts was identified. Both strands of the T-DNA code RNA. Hybridization of octopine TL-DNA against poly A RNA's present in two nopaline tumour-lines C58-S1 and BT37, and vice versa, reveals a minimum of two and possibly four transcripts common to both octopine and nopaline tumours. These transcripts originate from corresponding parts of the conserved region of the T-DNA and are of similar size.

Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Willmitzer L
Max-Planck-Institut für Züchtungsforschung, D-5000 Köln 30, FRG.
Simons G
Schell J
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1982-00-00
Pages
139-46
Language
English
Region
England
NLM ID
8208664
PMCID
PMC553008
External Links
PubMed source PMC Full Text View journal details
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