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PMID: 159209 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Plasmid pKC7: a vector containing ten restriction endonuclease sites suitable for cloning DNA segments.

Gene ·Vol. 7 ·No. 1 ·1979-09-00 ·Pages 79-82

Rao RN, Rogers SG

Abstract

Plasmid pKC7, a derivative of pBR322, specifies resistance to both ampicillin and kanamycin. The DNA of this small plasmid (5.8 kb) contains unique sites for insertion of DNA cleaved with ten different restriction endonucleases. A detailed restriction endonuclease cleavage map is presented. The utility of this plasmid for cloning is discussed.

MeSH Terms
Ampicillin/pharmacology Bacteriophage lambda/genetics,metabolism Cloning, Molecular DNA Restriction Enzymes/metabolism DNA, Recombinant/metabolism Kanamycin/pharmacology Penicillin Resistance Plasmids Substrate Specificity
Chemicals
DNA, Recombinant Kanamycin Ampicillin DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Rao R N
Rogers S G
Article Info
Journal
Gene
Abbr.
Gene
ISSN
0378-1119
DOI
10.1016/0378-1119(79)90044-1
Published
1979-09-00
Pages
79-82
Language
English
Region
Netherlands
NLM ID
7706761
Subset
IM
External Links
PubMed source DOI: 10.1016/0378-1119(79)90044-1 View journal details
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