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PMID: 2535532 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Multiple domains exist within the upstream activator sequence of the octopine synthase gene.

The Plant cell ·Vol. 1 ·No. 9 ·1989-09-00 ·Pages 925-36

Leisner SM, Gelvin SB

Abstract

It is known that a 16-base pair palindrome (ACGTAAGCGCTTACGT) located upstream of the ocs gene can activate a maize adh1 promoter in a transient expression system [Ellis et al. (1987). EMBO J. 6, 11-16; Ellis et al. (1987). EMBO J. 6, 3203-3208]. We have determined that this palindrome is also essential for ocs promoter activity in tobacco calli. In addition, sequences immediately adjacent to this palindrome, both 5' and 3', modulate its activity. The palindrome is sensitive to the differentiated state of the plant cells in which it resides; it is active in calli and the leaves of small shoots but is inactive in the leaves of rooted plants. We have tested upstream sequences from two other T-DNA genes that have homology to this palindrome for their ability to activate the octopine synthase promoter in tobacco calli. The upstream region from the mannopine synthase gene can activate the octopine synthase promoter, but an upstream region from the gene implicated in octopine and nopaline secretion cannot activate the promoter.

Related Genes
ocs
MeSH Terms
Agrobacterium tumefaciens/genetics Amino Acid Oxidoreductases/genetics,metabolism Base Sequence Cloning, Molecular DNA Molecular Sequence Data Plants, Toxic Plasmids Regulatory Sequences, Nucleic Acid Restriction Mapping Sequence Homology, Nucleic Acid Tobacco
Chemicals
DNA Amino Acid Oxidoreductases D-octopine dehydrogenase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Leisner S M
Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907.
Gelvin S B
References (18)
18 references, click to expand
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.1.9.925
Published
1989-09-00
Pages
925-36
Language
English
Region
England
NLM ID
9208688
PMCID
PMC159828
Subset
IM
External Links
PubMed source DOI: 10.1105/tpc.1.9.925 PMC Full Text View journal details
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