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PMID: 3561415 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Mutation of the c-fos gene dyad symmetry element inhibits serum inducibility of transcription in vivo and the nuclear regulatory factor binding in vitro.

Molecular and cellular biology ·Vol. 7 ·No. 3 ·1987-03-00 ·Pages 1217-25

Greenberg ME, Siegfried Z, Ziff EB

Abstract

In vitro mutagenesis of a 61-base-pair DNA sequence element that is necessary for induction of the c-fos proto-oncogene by growth factors revealed that a small region of dyad symmetry within the sequence element is critical for c-fos transcriptional activation. The same c-fos dyad symmetry element was found to bind a nuclear protein in vitro, causing a specific mobility shift of this c-fos regulatory sequence. An analysis of insertion and deletion mutants established a strict correlation between the ability of the dyad symmetry element to promote serum activation of c-fos transcription and in vitro nuclear protein binding. These experiments suggest that the DNA mobility shift assay detects a nuclear protein that mediates growth factor stimulation of c-fos expression. In vitro competition experiments indicate that the c-fos regulatory factor also binds to sequences within another growth factor-inducible gene, the beta-actin gene.

MeSH Terms
Animals Base Sequence DNA/genetics Gene Expression Regulation Humans Mice Mutation Nucleoproteins/genetics Proto-Oncogene Mas Proto-Oncogenes Transcription Factors/genetics Transcription, Genetic
Chemicals
MAS1 protein, human Nucleoproteins Proto-Oncogene Mas Transcription Factors DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Greenberg M E
Siegfried Z
Ziff E B
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.7.3.1217-1225.1987
Published
1987-03-00
Pages
1217-25
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC365195
Subset
IM
Grants
NCI NIH HHS · 5 F32-CA 07515-02 · United States
NIGMS NIH HHS · GM-30760 · United States
NCI NIH HHS · P30-CA-16087 · United States
Databases
GENBANK
M15429
External Links
PubMed source DOI: 10.1128/mcb.7.3.1217-1225.1987 PMC Full Text View journal details
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