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PMID: 6828386 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.

Nucleic acids research ·Vol. 11 ·No. 5 ·1983-03-11 ·Pages 1475-89

Dignam JD, Lebovitz RM, Roeder RG

Abstract

We have developed a procedure for preparing extracts from nuclei of human tissue culture cells that directs accurate transcription initiation in vitro from class II promoters. Conditions of extraction and assay have been optimized for maximum activity using the major late promoter of adenovirus 2. The extract also directs accurate transcription initiation from other adenovirus promoters and cellular promoters. The extract also directs accurate transcription initiation from class III promoters (tRNA and Ad 2 VA).

MeSH Terms
Animals Cattle Cell Nucleus/enzymology DNA-Directed RNA Polymerases/metabolism HeLa Cells/enzymology Humans Kinetics Magnesium/pharmacology Molecular Weight Operon Protease Inhibitors/pharmacology RNA Polymerase II/metabolism RNA, Neoplasm/isolation & purification RNA, Transfer/genetics Temperature Thymus Gland/enzymology Transcription, Genetic/drug effects
Chemicals
Protease Inhibitors RNA, Neoplasm RNA, Transfer RNA Polymerase II DNA-Directed RNA Polymerases Magnesium
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Dignam J D
Lebovitz R M
Roeder R G
References (20)
20 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.5.1475
Published
1983-03-11
Pages
1475-89
Language
English
Region
England
NLM ID
0411011
PMCID
PMC325809
Subset
IM
Grants
NCI NIH HHS · CA 16640 · United States
NCI NIH HHS · CA 23615 · United States
NIGMS NIH HHS · GM 06981 · United States
External Links
PubMed source DOI: 10.1093/nar/11.5.1475 PMC Full Text View journal details
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